培养基中几种重要营养元素对Anabaena sp. strain PCC 7120及Synechocystis sp. strain PCC 6803生长的影响
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国家自然科学基金项目(31570048); 中央高校基本科研业务费专项(2014PY003)


Effects of important nutrition elements in medium on the growth of Anabaena sp. strain PCC 7120 and Synechocystis sp. strain PCC 6803
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    摘要:

    配制KNO3、KHCO3、NaHCO3、FeNa2-EDTA及Na2S2O3营养盐培养液,并按一定浓度添加至液体或固体培养基中,获得含有不同营养素、不同浓度的液体及固体培养基,检测无机氮(NO-3)、无机碳(HCO-3)、Fe及Na2S2O3对集胞蓝细菌Synechocystis sp. strain PCC 6803和鱼腥蓝细菌Anabaena sp. strain PCC 7120细胞生长的影响。结果显示,集胞蓝细菌Synechocystis sp. strain PCC 6803在10 mmol/L硝酸盐生长较好,鱼腥蓝细菌Anabaena sp. strain PCC 7120在2 mmol/L 硝酸盐生长较好。不同浓度KHCO3及NaHCO3都会对蓝细菌的生长产生影响,可能是因为盐离子发挥了主要作用。此外,2种蓝细菌在10 mmol/L Na2S2O3中生长较好,不同浓度的铁盐对蓝细菌的生长产生较大影响,在10 μmol/L铁盐中生长最佳。

    Abstract:

    The cyanobacteria of Synechocystis sp. strain PCC 6803 and Anabaena sp. strain PCC 7120 are important models in basic and applied studies.Both species are conventionally cultured in BG11,although they show very distinct physiology.The effects of several nutrients in BG11 medium on the growth of Anabaena sp. strain PCC 7120 and Synechocystis sp. strain PCC 6803 were investigated.Results showed that the growth of Synechocystis sp. strain PCC 6803 and Anabaena sp. strain PCC 7120 was best at 10 mmol/L NO-3 and 2 mmol/L NO-3,respectively.Different concentrations of KHCO3 and NaHCO3 had different effects on the growth,indicating that both metal ions and HCO3- had a major role.For Na2S2O3  and Fe,the growth of Anabaena sp. strain PCC 7120 and Synechocystis sp. strain PCC 6803 was best at 10 mmol/L and 10 μmol/L,respectively.

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高良春,张巨源,王莉,陈雯莉.培养基中几种重要营养元素对Anabaena sp. strain PCC 7120及Synechocystis sp. strain PCC 6803生长的影响[J].华中农业大学学报,2016,35(6):

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  • 收稿日期:2015-12-08
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  • 在线发布日期: 2016-10-24
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