1株内生真菌的分离鉴定及其对铁皮石斛的促生作用
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国家自然科学基金项目(31460016); 广西自然科学基金项目(2014GXNSFBA118101,2015GXNSFBA139083); 广西农业科学院基本科研业务专项(2015YT80); 广西农业科学院科技发展基金项目(2015JZ14,2015YM04)


Isolation and identification of endophytic fungus Devriesia lagerstroem and its promoting effect on Dendrobium candidum growth
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    摘要:

    从不同植物的茎和叶中分离获得204株真菌,通过对白菜的致病性测定筛选获得1株能显著促进白菜生长的内生真菌菌株24L-4。结合形态学特征观察进行ITS-rDNA和18S-rDNA序列分析,对其进行鉴定,确定为Devriesia lagerstroem。该菌株对铁皮石斛组培苗的株高、叶宽、茎径和干质量分别较未接种对照处理增加33.3%、65.7%、27.3%和45.1%,显著高于对照处理。同时,盆栽试验表明:D. lagerstroem铁皮石斛盆栽苗的茎径、鲜质量和干质量分别较无接种对照增加104.0%、83.9%和114.7%,显著高于对照处理。显微镜观察发现:D. lagerstroem可定殖于铁皮石斛根部的外皮层和皮层。研究结果表明,D. lagerstroem对铁皮石斛具有明显的促生作用,可用于铁皮石斛菌肥的开发。

    Abstract:

    Two hundred and four fungal isolates were obtained from stems and leaves of different plants.One endophytic fungal isolate 24L-4 was selected by pathogenicity test on Chinese cabbage.Isolate 24L-4 was identified as Devriesia lagerstroem based on its morphological characteristics and sequences of internal transcribed spacer and 18S ribosomal DNA.Isolate 24L-4 was inoculated on Dendrobium candidum tissue cultures and pot seedlings,respectively,with non-inoculated seedlings as the control.The results showed that the seedling height,leaf width,stem diameter and dry weigh were significantly enhanced by 33.3%,65.7%,27.3% and 45.1%,respectively,in the tissue cultures.Similar results were observed in the pot experiment with increase percentages by 104.0%,83.9% and 114.7% for the stem diameter,fresh weight and dry weight,respectively.The hyphae and microscierotia-like structures of isolate 24L-4 were found in the root epidermal and cortex of D. candidum.These results indicated that isolate 24L-4 had excellent growth promoting effect on D. candidum.

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谢玲,张雯龙,蓝桃菊,陈艳露,覃丽萍,NARISAWA Kazuhiko,刘斌.1株内生真菌的分离鉴定及其对铁皮石斛的促生作用[J].华中农业大学学报,2016,35(3):83-88

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  • 收稿日期:2015-06-30
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  • 在线发布日期: 2016-04-29
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