两种内生真菌对大花蕙兰的共生效应比较
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国家自然科学基金项目(31170654);广东省农业攻关项目(2007A020200004-7)


Comparing the symbiotic effects of two endophytes on growth of Cymbidium hybridum
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    摘要:

    为探讨益生真菌对大花蕙兰组培苗生长的促进效应,从野生兰科植物根系分离的内生真菌中筛选出2株真菌菌株C2y1和S1g1,对其进行形态特征和ITS序列鉴定,分析2株真菌共生对大花蕙兰幼苗营养生长、矿质元素吸收以及叶绿素含量的影响,并检测了真菌在兰花根系中的定殖特点。结果表明:2株真菌能促进大花蕙兰植株根系生长、总生物量增长和叶绿素含量增加;C2y1相比于对照能显著促进大花蕙兰幼苗对N、P、K、Fe等矿质元素的吸收,尤其是菌根化苗Fe元素含量高出对照163.24%;菌株S1g1则显著促进植株对Ca、Mn、Zn 的吸收,处理植株的Ca和Zn含量高出对照62.00%和68.09%;形态结构和ITS序列分析鉴定表明C2y1为兰科丝核菌类瘤菌根菌属(Epulorhiza sp.)真菌,S1g1为黄丝菌科Cephalotheca属真菌。显微结构分析表明,C2y1菌丝可侵入兰根皮层细胞,并形成典型的菌丝结,而S1g1仅在兰根的根被组织聚集。菌根化可有效促进大花蕙兰组培苗的营养生长和矿质元素吸收,而不同种类的真菌对其促进效应表现出一定程度的互补性。

    Abstract:

    The vegetative growth of Cymbidium hybridum plantlets could be promoted through artificial mycorrhizae. In this study, two endophytes(C2y1 and S1g1) obtained from roots of wild orchids exhibited obvious growth-promoting effects on the C. hybridum plantlets through increasing root length,biomass and chlorophyll content.Furthermore,isolate C2y1 increased the N,P,K,Fe contents of the symbiotic plantlets.Especially the Fe content of the treated plants increased by 163.24% compared with the control.Isolate S1g1 significantly increased the accumulation of Ca,Mn,Zn (by 62%,32%,and 68.09%,respectively) in symbiotic plantlets compared with the control.Combining with internal transcribed spacer sequence (ITS) and the morphological characteristics,isolate C2y1 and S1g1 was identified as Epulorhiza sp. and Cephalotheca sp.,respectively.Microscopic studies showed that hyphae of isolate C2y1 infected the root cortex cells and formed typical orchid mycorrhizae,whereas hyphae of isolate S1g1 aggregated in the host velamen cells.It is indicated that artificial mycorrhization can effectively promote the vegetative growth and mineral element absorption for Cymbidium plantlets.Different fungi exhibit some degree of complementary promoting effects.

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刘舒,陈春黎,刘敏,廖庆刚,赵小兰.两种内生真菌对大花蕙兰的共生效应比较[J].华中农业大学学报,2016,35(1):43-49

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  • 收稿日期:2015-03-01
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  • 在线发布日期: 2015-12-23
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