Abstract:The vegetative growth of Cymbidium hybridum plantlets could be promoted through artificial mycorrhizae. In this study, two endophytes(C2y1 and S1g1) obtained from roots of wild orchids exhibited obvious growth-promoting effects on the C. hybridum plantlets through increasing root length,biomass and chlorophyll content.Furthermore,isolate C2y1 increased the N,P,K,Fe contents of the symbiotic plantlets.Especially the Fe content of the treated plants increased by 163.24% compared with the control.Isolate S1g1 significantly increased the accumulation of Ca,Mn,Zn (by 62%,32%,and 68.09%,respectively) in symbiotic plantlets compared with the control.Combining with internal transcribed spacer sequence (ITS) and the morphological characteristics,isolate C2y1 and S1g1 was identified as Epulorhiza sp. and Cephalotheca sp.,respectively.Microscopic studies showed that hyphae of isolate C2y1 infected the root cortex cells and formed typical orchid mycorrhizae,whereas hyphae of isolate S1g1 aggregated in the host velamen cells.It is indicated that artificial mycorrhization can effectively promote the vegetative growth and mineral element absorption for Cymbidium plantlets.Different fungi exhibit some degree of complementary promoting effects.