四川白鹅RPS27a和SDC2基因差异表达
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国家自然科学基金项目(31201798);高等学校博士学科点专项科研基金项目(20105103120003)


Differential expression profiling of RPS27a and SDC2 genes in Sichuan white goose
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    摘要:

    为阐明鹅不同组织和卵泡中RPS27a和SDC2基因的表达规律,明确其在卵泡发育过程中的作用,应用实时荧光定量PCR检测四川白鹅17种组织及卵泡中RPS27a和SDC2基因的相对表达量。结果表明:四川白鹅卵巢组织中RPS27a的表达量最高(P<0.05),而小脑中的表达量最低;肝脏中SDC2的表达量最高(P<0.05),SDC2在腿肌和胸肌中的表达量最低。在等级卵泡中,小黄卵泡RPS27a和SDC2表达量均显著低于小白卵泡;F2卵泡中RPS27a的表达量显著高于F5和F3;F2卵泡中SDC2的表达量是小白卵泡的2.05倍,且显著高于F5、F4和F3卵泡,但与F1卵泡差异不显著;在排卵后卵泡中,RPS27a和SDC2的表达量呈显著下降趋势。RPS27a和SDC2在所检测的组织中均有表达,但具有组织特异性的特点;RPS27a和SDC2可能对维持鹅卵巢功能具有重要调控作用,且RPS27a和SDC2在排卵后卵泡中显著降低可能对排卵后卵泡的退化消失具有促进作用。

    Abstract:

    To elucidate the possible function of the RPS27a and SDC2 genes during ovarian development,relative expression of the RPS27a and SDC2 in 17 tissues and follicles of Sichuan white geese were determined by quantitative real-time PCR.The highest RPS27a expression was detected in the ovarian stroma (P<0.05) and the lowest was in the cerebellum.The highest expression of SDC2 was in the liver (P<0.05),and the lowest was in the breast and thigh muscle (P<0.05).In the ovarian hierarchical follicles,the RPS27a and SDC2 mRNA was significantly lower in the small yellow follicles (SYF) than that in the small white follicles (SWF).The RPS27a expression of F2 was significantly higher than that of F5 and F3.The SDC2 of F2 was as 2.05 folds as that of SWF,and was significantly higher than that of F5,F4 and F3.The SDC2 mRNA was not significantly different between F2 and F1.During the postovulatory,the RPS27a and SDC2 expression decreased significantly.The lowest expression of RPS27a and SDC2 was observed in POF3 (P<0.05).The RPS27a and SDC2 are expressed ubiquitously and tissue-specifically,suggesting that they may play an important role in maintaining the ovarian function and they may also play important roles in accelerating the process of ovarian development and postovulatory follicles degenerating.

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何珲,姜冬梅,马容,徐麒麟,陈咨余,康波.四川白鹅RPS27a和SDC2基因差异表达[J].华中农业大学学报,2015,34(6):89-93

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  • 收稿日期:2015-05-04
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  • 在线发布日期: 2015-10-13
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