This experiment employed four methods to test content of intramuscular fat in various types of pork.The coefficients of correlation between fresh sample extraction and near infrared spectroscopy method,fresh sample extraction and Soxhlet extraction method,Soxhlet extraction method and near-infrared spectroscopy method,were 0.989,0.978,and 0.981,respectively.However,the coefficient of correlation is lower than 0.9 between the marbling score method and the other methods.Through comparison of the four methods,it showed that the test results by Soxhlet extraction method was significantly lower than fresh sample extraction and near-infrared spectrum method (P<0.05 )，and there was no significant difference between fresh sample extraction and Near-infrared spectrometry method (P>0.05).It had significant differences between marbling score method and other methods,suggesting that it can only be used as an assistant method.Several different varieties were tested by the fresh sample extraction and the near-infrared spectroscopy method,but the result showed that there was no significant difference between the two methods (P>0.05).Therefore,fresh sample extraction is still the most accurate and effective classical method for determination of intramuscular fat pork,while the near infrared spectroscopy can be used as a rapid detection method,which is simply,quickly and accurately.