In this study,a survey was carried out to identify Meloidogyne species from rhizosphere of vegetables in Shenzhen City by means of morphology, isozymes and molecular analysis. The results showed that among 121 samples of roots and rhizosphere soil from 13 different vegetable bases in Shenzhen City, Meloidogyne were found in 74 samples from 11 different fields, and the incidence rate of Meloidogyne was 61.16%. Seventy six populations of Meloidogyne were purified, and 4 species were detected and identified, including 69 populations of M.incognita, 3 populations of M.javanica, 1 population of M.arenaria and 3 populations of M.enterolobii. At the same time, mixed populations of M.incognita and M.enterolobii existed in 2 samples. In conclusion, Meloidogyne distribute widely in vegetable fields in Shenzhen City,and M.incognita is the dominated species.