Based on the porcine SIRT1 mRNA sequence from GenBank，primers of porcine SIRT1 gene were designed and coding region of SIRT1 gene was amplified.The epitope sequences located on the rear section of SIRT1 protein were then inserted into pET-28a(+) by Ω-PCR.The SIRT1 protein was expressed in E.coli,purified and its polyclonal antibody was produced by immuning rabbit，the protein weight is about 45 ku and the antibody titers is about 212.The results showed that antibody is capable of detecting the porcine SIRT1 protein specifically and provided a new tool for studies of the cellular path way involving in porcine SIRT1.