A pot experiment was conducted to study the application effects of acid soil conditioner on the growth, seed yield of winter oilseed rape and soil pH. The results showed that acid soil conditioner could enhance the growth and improve seed yield of winter oilseed rape by increasing pod numbers per plant, ratio of effective seed setting and seed numbers per pod. Compared with the control, the average height of plant treated with acid soil conditioner at seedling stage, stem-elongation stage, early-flowering stage and pod stage increased 19.6%, 12.5%, 12.2% and 11.6%, respectively. The SPAD values of leaf were increased with the average of 0.5%, 6.3%, 9.3% and 10.2% at those four stages. At mature stage, the primary branches numbers, pod number per plant, ratio of effective seed setting, seed number per pod were increased with the average of 41.7%, 42.7%, 10.5% and 20.9%, respectively. The average increase rate of yield was 25.4%. The soil conditioner could enhance soil pH. Compared with the control, the soil pH values were increased on average with 0.13, 0.03, 0.11 and 0.38 unit at seedling stage, stem-elongation stage, early-flowering stage and pod stage, respectively. It is indicated that the acid soil conditioner can be applied to improve the growth of oilseed rape and soil pH under acid soils. 