分子标记辅助选择改良水稻恢复系R1005的褐飞虱抗性
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国家“863” 计划项目(2012AA101101)和湖北省研究与开发计划项目(2011BBB003)


Improving the brown planthopper resistance of rice restorer line R1005 with molecular marker-assisted selection
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    摘要:

    以抗褐飞虱材料B5(携带Bph14和Bph15基因)为供体亲本,水稻优良恢复系R1005为受体亲本,通过杂交、回交和分子标记辅助选择技术,将Bph14和Bph15基因同时导入到R1005中,经过农艺性状选择和褐飞虱抗性鉴定,选育出3个携带Bph14和Bph15基因的纯合株系CY11711-14、CY11712-5和CY11714-100。苗期褐飞虱抗性鉴定结果表明,3个株系对褐飞虱表现高抗水平,田间生育期观察、主要农艺性状考查及稻米品质分析表明,株系CY11712-5和CY11714-100与受体亲本R1005没有显著差异,可以代替R1005用于抗褐飞虱恢复系使用。以3个育成株系为父本与3个不育系Q2A、天丰A和川农1A配制杂交组合进行苗期褐飞虱抗性鉴定,结果显示Bph14、Bph15基因在杂合状态下表现不抗褐飞虱。结果表明Bph14和Bph15基因在本试验中不表现显性效应。

    Abstract:

    Brown planthopper (BPH) is one of the serious pests in rice production of China.The breeding of rice varieties with resistance against BPH is an effective way to control this pest.Rice line B5 carrying two brown planthopper resistance genes Bph14 and Bph15 was used as donor parent to cross and backcross with recipient parent R1005 with molecular marker-assisted selection.Three lines (CY11711-14,CY11712-5 and CY11714-100) carrying Bph14 and Bph15 genes were bred through identifying the BPH resistance and evaluating main agronomic traits.The results showed that all three bred lines were highly resistant to BPH.The results of evaluating yield,growth duration,main agronomic and quality traits showed that CY11712-5 and CY11714-100 were similar as the recipient parent R1005.Both nine hybrids from crossing three bred lines with the Q2A,Chuannong 1A,Tianfeng A and three bred lines were not resistant angainst BPH,indicating that Bph14 and Bph15 genes did not have the dominant effect in the experiment.

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闫成业,MAMADOU Gandeka,朱子建,牟同敏.分子标记辅助选择改良水稻恢复系R1005的褐飞虱抗性[J].华中农业大学学报,2014,33(05):8-14

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  • 收稿日期:2013-06-13
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