The Streptomyces lividans genome carries two gene clusters encoding the biosynthetic pathways of actinorhodin and undecylprodigiosin.However neither antibiotic is produced in S.lividans under the conditions of common laboratory growth.Malonyl-CoA as precursor was needed in the biosynthesis of both compounds catalyzed by the committed enzyme acetyl-CoA carboxylase (ACC).This study was aimed to clone the acc genes from Escherichia coli and introduce them into S.lividans to investigate their effect on the production of actinorhodin and undecylprodigiosin.The E.coli ACC subunits are encoded by 4 acc genes.These acc genes were cloned with adding ermE promoter to each through overlapping extension PCR to gain 4 recombined genes.The recombinant genes were then cloned into pSET152 successively to gain a heterologous expression plasmid.The constructed plasmid was transferred into S.lividans by conjugation,and the antibiotic production in exconjugants was examined.The exconjugants could not only produce actinorhodin but also showed a prominent increase in the production of undecylprodigiosin.acc from E.coli can improve the production of pre-existing antibiotics and activate the expression of silent gene clusters as well in S.lividans.