甲基茉莉酸和草酸诱导油菜BN10基因的表达
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湖北省自然科学基金项目(2009CDA160)和华中农业大学自主科技创新基金项目(2010QC031)


Gene expression of oilseed rape BN10 induced by methyl jasmonate and oxalic acid
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    摘要:

    利用实时荧光定量PCR方法,观察油菜BN10基因在根、茎、叶中的表达差异,并分析甲基茉莉酸(MeJA)和草酸(OA)对该基因的诱导表达的动态变化。结果表明:BN10基因在油菜根、茎、叶中均能表达,其中在根中的表达量最高,茎中的表达量次之,在叶中的表达量最低,且在抗病毒品种苏油1号中的表达量高于感病品种中双9号;BN10的表达受MeJA和OA的诱导影响。MeJA诱导处理感病品种中双9号叶片后,BN10基因表达先升后降,在24 h时表达量最高,为诱导前的2.58倍,之后有所下降,但诱导后的表达量始终高于诱导前,72 h时的表达量仍为诱导前的1.13倍; OA诱导处理中双9号后,BN10表达量也是先增加后降低,在12 h时达到最大值,为诱导前的2.5倍,72 h时下降至最低,仅为诱导前的25%。MeJA诱导处理抗病品种苏油1号后,BN10的表达趋势与中双9号相似,但各时间点的表达量均高于中双9号; OA诱导处理苏油1号后,对BN10的诱导更强烈,表达量在6 h时即达到峰值,为诱导处理前的3.05倍,之后逐渐下降。

    Abstract:

    Using real-time quantitative PCR, the BN10 gene expression in roots, stems and leaves of rape was detected. Its expression after the treatment with methyl jasmonate (MeJA) and oxalic acid (OA) was also analyzed. The results indicated that the expression of the BN10 gene was detected in roots, stems and leaves of rape with the highest expression level in roots, whereas the lowest expression level in leaves. The expression level in the antiviral variety Suyou No.1 was higher than that in the susceptible variety Zhongshuang No.9. The BN10 gene expression was induced by MeJA and OA in a similar mode. BN10 was significantly induced after the treatment with OA and MeJA for 12 h and 24 h in Zhongshuang No.9 respectively. The expression level was 2.50-folds and 2.58-folds higher than that before the induction, followed by a decrease. After induction for 72 h, the gene expression level in the treatment of OA was 75% lower than that of the untreated control, whereas was still 1.13-folds higher in the treatment of MeJA than that of the untreated control. The BN10 gene expression in Suyou No.1 was similar to that in Zhongshuang No.9 induced by MeJA and OA excepted for that its expression level was higher than that in Zhongshuang No.9. BN10 was significantly induced after the treatment with OA for 6 h. The expression level was 3.05 folds higher than that before the induction, followed by a decrease.

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肖春芳,侯明生,蔡丽.甲基茉莉酸和草酸诱导油菜BN10基因的表达[J].华中农业大学学报,2012,31(5):593-597

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  • 收稿日期:2011-12-30
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