HJ-37 was developed through backcross and marker-aided selection by using japonica variety Nipponbare as donor parent and indica variety Zhenshan 97B as recurrent parent.F2 seeds from crossing HJ-37 with Zhenshan 97B were used to select the recombinant plants with SSR markers.Then,the homozygous seeds from the recombinant plants were used to test the plant regeneration frequency.Meanwhile,SSR was utilized to map the recombinant sites.Finally,the effect of this QTL was verified.Average of plant regeneration frequency and t value of plant 10 is 53.31% and 4.897 6.This data shows plant 10 is obviously improved in PRF.Meanwhile,the distance of QTL was narrowed to the segment between SSR markers RM3289 and RM6295.The result will be useful for isolating and cloning the gene of high frequency of plant regeneration.