菜籽粕替代豆粕对异育银鲫非特异性免疫机能的影响
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国家科技支撑计划项目(2007BAD37B02)、武汉市科技攻关计划项目(2002200513204)和淡水生态与生物技术国家重点实验室开放课题(2003FB01)


Effects of soybean meal replacement by rapeseed meal on the non-specific immune function of gibel carp,Carassius auratus gibelio
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    摘要:

    试验鱼按投喂饲料的不同分为5组,其中对照组(RM0)以豆粕和鱼粉为基础蛋白源,试验组分别以双低菜籽粕(DLRM)和普通菜籽粕(CRM)等氮替代对照组中50%(DLRM50,CRM50)和100%(DLRM100,CRM100)的豆粕蛋白。分别在投喂菜籽粕后的当天与第14、28、42、56天取样,测定异育银鲫血液白细胞和头肾吞噬细胞的吞噬活性、血清溶菌酶活性、血清补体(C3、C4)含量等免疫指标的变化。结果表明:在投喂菜籽粕后28 d内,各项免疫指标变化不明显(P>0.05);在第42天,DLRM100组、CRM100组的血液白细胞和头肾吞噬细胞的PP、PI,CRM100组的血清溶菌酶活性,DLRM100组的C4含量,CRM100组的C3、C4含量均显著低于对照组(P<0.05);在第56天,DLRM100、CRM100 组的各项免疫指标都显著低于对照组;在整个试验过程中,DLRM50、CRM50组的各项免疫指标和对照组都没有显著的差别。

    Abstract:

    Five experimental diets for gibel carp,Carassius auratus gibelio,were formulated to contain various percentages of common rapeseed meal (CRM) or double low rapeseed meal (DLRM) as a partial replacement for soybean meal.RM0(with 0% CRM and DLRM) was formulated as the control group.DLRM50 (CRM50),DLRM100(CRM100) were formulated to isonitrogenously replace 50% and 100% of soybean meal protein in RM0 with DLRM (CRM),respectively.The non-specific immune parameters including phagocytic activity (PP,PI) of blood leucocytes and head kidney phagocytes,serum lysozyme activity,serum complement concentrations of C3 and C4 were evaluated at every 14-day intervals.During the initial 28-day period,only PP of blood leucocytes in CRM100 group was significantly lower than that in RM0 treatment(P<0.05).From the 42th day on,both PP and PI of blood leucocytes and head kidney phagocytes in DLRM100 and CRM100 treatments were all significantly lower than those in RM0 treatment (P<0.05).On the 56th day,serum lysozyme activity in CRM100 and DLRM100 groups was significantly lower than that in RM0 group(P<0.05).From the 42th day,serum complement concentrations of C3 or C4 in DLRM100 and CRM100 treatments were significantly lower than those in RM0 group(P<0.05).The immune parameters in DLRM50 and CRM50 treatments were not significantly changed compared with those in RM0 group (P>0.05).However those in CRM50 group gradually reduced with time.We concluded that the effects on non-specific immune function might cause by antinutritional factors such as glucosinolates,sinapin,phytic,tannin,crude fibre in the rapeseed meal.

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赵 飞,吴志新,陈孝煊,姜 兰.菜籽粕替代豆粕对异育银鲫非特异性免疫机能的影响[J].华中农业大学学报,2012,31(2):237-242

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