香蕉枯萎病菌2个生理小种多聚半乳糖醛酸酶活性的比较
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国家自然科学基金项目(30671349)和公益性行业(农业)科研专项(200903049-05)


Comparison of polygalacturonase produced by Fusarium oxysporum f.sp. cubense race 1 and race 4
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    摘要:

    用香蕉枯萎病菌4号生理小种(Foc4)接种巴西香蕉,香蕉枯萎病菌1号生理小种(Foc1)接种粉蕉,均能检测到PG、PMG、PGTE、PMTE和Cx 5种细胞壁降解酶活性,其中PG活性均最高。在7种不同碳源培养条件下,Foc4的菌丝干质量都比Foc1的大,说明Foc4的生长更快,能更适应多种营养条件。在PG诱导方面,有柑桔果胶或PGA存在时,2个生理小种的PG活性明显加强。在葡萄糖或CMC作为唯一碳源时,2个生理小种的PG活性很低。以香蕉维管束组织培养2个生理小种后发现,Foc4的PG活性比Foc1高。以粉蕉维管束组织培养2个生理小种后发现,Foc1的PG活性比Foc4高。以柑桔果胶作为碳源,并改变不同的氮源,在7种氮源培养条件下,Foc1和Foc4均能产生PG活性,但所产生的PG活性比不加氮源所产生的PG活性低。

    Abstract:

    All of the five cell wall-degrading enzymes,including PG,PMG,PGTE,PMTE and Cx,could be found in Brazil banana inoculated by banana wilt fusarium race 4 and Chinese banana inoculated by banana wilt fusarium race 1.PG activity was the highest.Under the condition of being cultured with seven different carbon sources,the dry weight of mycelium biomass from Foc 4 was lager than that from Foc 1,which means Foc 4 grows more quickly and is more adaptable.With the presence of citrus pectin or PGA,the two strains significantly induced PG activity.When using CMC or glucose as the sole carbon source,the PG activity of the two strains was very low.The PG activity of Foc 4 was higher than that of Foc 1 in the musa vascular tissue culture,while the PG activity of Foc 4 was lower than that of Foc 1 in the Chinese banana vascular tissue culture.With citrus pectin as a carbon source,both Foc 1 and Foc 4 can produce PG activity,but they produced lower PG activity than they did without nitrogen source.

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董章勇,王振中.香蕉枯萎病菌2个生理小种多聚半乳糖醛酸酶活性的比较[J].华中农业大学学报,2012,31(2):204-207

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  • 收稿日期:2011-06-27
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