Rhus chinensis Mill (RCM)， Rheum palmatum Linn (RPL)， Rhizoma polygoni Cuspidati (RPC)，and Punica granatum Linn (PGL)，were first ground into 5-10 μm superfine powder with ultra-pulverization.Then they were mixed one by one based on chessboard experimental method to get four kinds of compounds of Chinese herbs.To study inhibitation activity of the compounds，the minimum inhibitory concentration （MIC） and the minimum bactericidal concentration （MBC） of single drug and the triple compounds against nine strains of pathogenic bacteria from eels were measured with agar dilution method.The results showed that four kinds of Chinese herbs had certain antibacterial effects on nine bacteria.RCM had the best antibacterial effect，followed by PGL and RPL，and RPC had little antibacterial effect.Four triple combinations had synergism antibacterial effects on pathogenic bacteria，and the value of FIC was from 0.38 to 0.76.Antibacterial synergistic effect of prescription No.1 was the best，whose value of FIC is less than or equal to 0.5 against nine strains of pathogenic bacteria.The inhibitory concentration of each triple compounds reduced at least 79%，and the cost calculated in aquaculture production was lower than single Chinese herbs at least 45%.