Nematodes of the genus Bursaphelenchus collected from Guangdong Province were identified by using morphology, rDNA-ITS-RFLP and rDNA-ITS-PCR-sequencing. B.fungivorus and B.doui were isolated from a growing substrate for Ficus microcarpa in Panyu and wood packaging material in Dongguan respectively. B.fungivorus was a new geographic record species in China, and B.doui was a new geographic record species in Chinese Mainland.