Abstract:A new venom allergen-like proteins,named Dd-vap-2,was isolated from Ditylenchus destructor by RT-PCR and RACE. The full length cDNA of Dd-vap-2 consists of 1 150 bp, with a 912 bp ORF encoding a 303 amino acid protein (GenBank accession No. GU370352).The putative protein with the oretical molecular weight was 31.8 ku in weigh and its pI was 6.76. The sequence analysis indicated that this gene was classified as a member of cysteine-rich secretory protein, CRISP and has a 21 amino acids long signal sequence at N terminal. Phylogenic analysis suggested that Dd-vap-2 had a close homology to Globodera rostochiensis and Heterodera glycines.