To understand anti-infectious response to bacteria in the Asian yellow pond turtle (Mauremys mutica),a full length cDNA library was constructed for it by SMART technique experimentally infected with Serratia marcescens.Firstly,the double-strand cDNA was synthesized using SMART TM PCR cDNA systhesis kit.Second,the ds cDNA was separated into two parts based on the size distribution of amplified ds cDNA by agarose gel size fractionation.The part shorter than 500 bp was discarded and the other one longer than 500 bp was ligated to the pGEM-T vector.The ligation mixture was transformed into E.coli JM109 by electroporation.The cDNA library contained 1.8×105 independent clones with DNA inserts of 0.5-3.0 kb.The recombination rate was 90.3%.We sequenced 80 cDNA clones about 1 kb and most of the genes were found the first time in reptiles.We classified these clones in functions with 9 in immunity,6 in cell signaling,8 in catalytic activity,2 in sugar/glycolysis metabolism,1in transport metabolism,and 2 in cell structure.The successfully constructed cDNA library will be essential for rapid isolation of differentially expressed genes related to Serratia marcescens infection and useful for understanding the anti-infectious molecular mechanism in the Asian yellow pond turtle.