根际细菌9个菌株对南方根结线虫的盆栽防效
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广西自然科学基金项目(桂科基0991017,0575035)资助


Control Effect of 9 Strains of Rhizobacteria to Meloidogyne incognita of Tomato Plant on Potted Experiment
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    摘要:

    通过盆栽试验,观察了土壤细菌9个菌株对番茄上南方根结线虫的生物防治效果。结果表明:供试菌株能够降低番茄线虫的根结指数,且对番茄生长有一定的促进作用。119号菌株处理30 d后,防治效果最好,平均防效达57.75%; 处理60 d后,平均防效达55.88%。333号菌株处理30 d后,番茄植株生长最好,株高达43.00 cm,株鲜质量达11.17 g,分别比对照提高了37.25%和76.46%。对单个菌株防效比较好的菌株进行初步混用试验,结果表明:部分菌株混用的防治效果不太理想,但对番茄的株高和鲜质量有促进作用,且与对照差异显著。通过16S rDNA序列分析,并结合形态学和生理生化特征试验,初步鉴定119号菌株为巨大芽孢杆菌 Bacillus megaterium。

    Abstract:

    Through the potted experiments of tomato in greenhouse, the biocontrol ability of 9 strains of rhizobacteria to root knot nematodes(Meloidogyne incognita) was studied. The results showed that these strains could reduce the formation of the galls: the control efficiency of strain 119 was the greatest at 57.75% after 30 days, and 55.88% after 60 day. These 9 strains could also promote the growth of tomato plants too: strain 333 showed best growth promotion ability, the plant height and mass were 43.00 cm and 11.17 g respectively, and were increased by 37.25% and 76.46% compared to those of CK. However, the application of a mixture of the strains did not provide significant disease suppression compared to the application of a single strain, but could significantly promote the growth of tomato plants. Strain 119 was classified as Bacillus megaterium,based on the analysis of their 16S rDNA sequences, and the morphological and biochemical tests.

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黄金玲,刘志明,陆秀红,刘纪霜,秦碧霞,乔丽娅.根际细菌9个菌株对南方根结线虫的盆栽防效[J].华中农业大学学报,2010,29(6):700-703

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  • 收稿日期:2010-04-06
  • 最后修改日期:2010-06-28
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