Abstract:The genomic DNA of Ormosia henryi Prain was digested by MseⅠand ligated with adapters.After PCR amplification,the fragments ranging from 500 to 1 000 bp were isolated,purified and hybridized with biotin-labeled probes (GA)12 and (AAG)8.Fragments containing simple sequence repeats (SSR) were enriched using magnetic beads coated with streptavidin,then cloned into pMD19-T vectors and transformed into E.coli to construct SSR genomic DNA library.78 positive clones were screened with PCR method.After sequenced,24 microsatellite sequences were identified and 9 pairs of SSR primers of Ormosia henryi Prain were developed successfully.