青鱼肠道出血症病原菌的分离与鉴定
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国家大宗淡水鱼产业技术体系建设专项资金(nycytx-49-16)和农业部公益性行业科研专项(200803013)资助


Isolation and Identification of Pathogen from Enteric Septicemia of Black Carp (Mylopharyngodon piceus)
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    摘要:

    从患典型肠道出血症的青鱼肝脏中分离到1株致病菌BCB01,人工腹腔注射感染试验可复制出与自然发病相似的症状。单克隆菌落的Biolog系统鉴定结果表明,菌株BCB01为嗜水气单胞菌(Aeromonas hydrophila)。进一步的16S rRNA基因测序与系统进化树分析结果表明,菌株BCB01与嗜水气单胞菌(EF645799)的亲缘关系最近,其相似性为99%。药物敏感性试验结果显示,该菌株对大多数试验药物敏感,其中对复达欣、头孢西丁、先锋必等11种药物高度敏感。

    Abstract:

    The dominant bacterial strain,designated as BCB01,was isolated from the kidney of enteric septicaemia of black carp Mylopharyngodon piceus.Artificial intraperitoneal injection with the bacterial suspensions to healthy black carp could reproduce the diseased symptoms as occurred in wild.Identification of the pathogen by Biolog system showed that the isolated causative pathogen BCB01 was Aeromonas hydrophila.According to their 16S rRNA gene sequence and molecular phylogenetic dendrogram,the strain BCB01 had close relationship to Aeromonas hydrophila with a similarity of 99%.The susceptibility test to antibiotics demonstrated that the isolate strain BCB01 was susceptible to most of the tested antibiotics,especially highly susceptible to 11 kinds of antibiotics,such as fortum,cefoxitin,cefobid and so on.

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张 波,曾令兵,罗晓松,徐 进,肖 艺,周 勇.青鱼肠道出血症病原菌的分离与鉴定[J].华中农业大学学报,2010,29(5):607-612

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  • 收稿日期:2010-03-26
  • 最后修改日期:2010-06-30
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