Effects of plant hormones,sucrose and active carbon contained in the culture medium on rooting of regenerated shoots were studied using regenerated shoots of Anemone flaccida as explants.The results showed that 1/2 MS supplemented with NAA 0.5 mg/L and IBA 0.5 mg/L,30.0 g/L sucrose as carbon sucrose and added with active carbon 1.0 g/L was suitable for rooting of A.flaccida.Under this medium the rate of rooting can reach up to 93.9%,number of root was 10.5 per explant and length of root was 1.73 cm (the longest).After culturing for 55 d root was more thick and less brown.Rooting system of A.flaccida can be optimized by increasing quantity and quality of regenerated root,thus increasing survival rate of transplanting.