The over-expression and RNAi constructs of AtGLB1were constructed and transformed into Arabidopsis plants with floral dipping method.Northern blot analysis of AtGLB1 expression in homozygous T3 lines of transgenic Arabidopsis showed that AtGLB1mRNA were not identified in lines expressing the AtGLB1RNAi construct,while lines over-expressing AtGLB1 showed a substantially high level of AtGLB1 mRNA compared with the wild-type.The study laid foundation for further researches on the function and application of AtGLB1 gene in agriculture.