Using leaves,petioles and roots of Saussurea involucrate as explants,the effect of plant hormones on callus induction in MS medium was studied.The results showed that the medium containing NAA was better than other media.Comparing with whole explants,callus induction of leaves and roots,petioles and roots differed significantly.The inducing effect of roots,leaves and petioles was 100%,98% and 44%,respectively.Using the combination of NAA and 6-BA,callus induction of leaves,petioles and roots differs significantly as well.The induction effect of leaves,petioles and roots was 98%,64% and 23%,respectively.