Abstract:n order to optimize the tissue culture conditions of Indica rice,the effects of trace elements on calli inducing and browning rate of Indica Yuetai B (YTB) were studied based on N6 culture medium.The results showed that inducing rate of calli wasn’t affected significantly by the concentration of minor elements,whereas the browning rate was the strongly affected.The browning rate was the lowest,which was 10.35%,on the 1.2×concentration of trace elements medium.More importantly,the status of calli was compact with good viability.The browning rate of 0.8×concentration was intermediate with 26.15%,whereas that of 1.0×concentration treat was the highest with 44.55%.