间接竞争ELISA检测大豆11S球蛋白和7S伴球蛋白
CSTR:
作者:
中图分类号:
Q503
基金项目:
浙江省重大科技攻关项目(2004C12610)资助
Quantitative Analysis of Soybean 11S Globulin and 7S Conglycinin by Indirect Inhibiting ELISA Method
Author:
- 摘要 | |
- 访问统计 | |
- 相似文献 [20] |
- 引证文献 | |
- 文章评论
摘要:
采用酸沉淀、亲和层析(ConA-Sepharose 4B)等手段,成功地分离纯化了大豆11S球蛋白和7S伴球蛋白,SDS-PAGE检测表明2种蛋白纯度分别达到95%和90%。将纯化的11S球蛋白和7S伴球蛋白分别免疫大白兔获得抗血清,并建立起间接竞争ELISA检测大豆豆粕及其深加工产品中11S和7S抗原蛋白含量的方法。
Abstract:
An indirect inhibiting ELISA method for the quantification of 11S(glycinin) and 7S protein(beta-conglycinin) was developed.First,soybean 11S globulin and 7S conglycinin were separated successfully by acid precipitation and affinity chromatography using a
引用本文
彭楠,刘建峰,梁运祥,葛向阳.间接竞争ELISA检测大豆11S球蛋白和7S伴球蛋白[J].华中农业大学学报,2007,26(5):
复制
文章指标
- 点击次数:1433
- 下载次数: 113
- HTML阅读次数: 0
历史
文章二维码
