Callus Induction and Bud Formation of Rubia yunnanensis (Franch.) Diels
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摘要:
通过不同外植体、基本培养基、暗培养、激素对小红参愈伤组织诱导和分化的试验研究,结果表明诱导小红参愈伤组织产生的最佳条件为:以真叶和顶芽(或侧芽)为外植体,在MS BA 0.2 mg/L NAA 3.0mg/L培养基上,进行暗培养,愈伤诱导率达95%。芽形成的愈伤组织组织在MS BA 5.0 NAA 1.5~2.0中均能分化出幼苗,分化率达100%,但叶形成的愈伤组织未能分化出苗。
Abstract:
The effect of different plantlet,basic medium,dark culture,hormone combination and densities on the formation and differentiation of callus ofEucalyptus smithiiL was studied.It was found that callus form well in the dark using true leaf and bud as plantle