摘要
土壤微生物群落结构失衡是番茄连作障碍发生的重要因素之一。为探明番茄连作障碍发生机制,推动番茄产业的可持续发展,本文对番茄连作障碍土壤微生物群落的变化特征及其重塑技术研究进展进行了综述。番茄连作土壤中微生物多样性下降,土壤微生物群落结构由“细菌型”转变为“真菌型”,是番茄连作障碍发生的主要原因;轮作与间套作、嫁接、土壤消毒和施用有机肥均可重塑连作番茄土壤的微生物群落结构,有助于减轻连作障碍危害,尤其轮作与间套作豆科作物更有利于减轻番茄连作障碍危害。迄今,研究发现节杆菌属(Arthrobacter)、芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas)、土壤红色杆形菌属(Solirubrobacter)细菌以及木霉菌属(Trichoderma spp.)真菌等微生物是有助于克服番茄连作障碍的功能微生物。开发与有机肥结合的生物有机肥,用于重塑农田土壤微生物群落的方法是一项极具开发前景的生物防控连作障碍发生的技术措施,但如何保障生物有机肥应用于不同环境中,功能微生物的定殖、存活与大量繁殖,是该项技术亟待解决的重要瓶颈问题。
番茄(Solanum lycopersicum L.)是茄科茄属的1年生草本植物,含有多种维生素,营养价值丰富,受到人们的广泛喜爱。联合国粮农组织统计数据显示,2021年番茄全球总产量达1.89亿t,在蔬菜作物中位居第一,我国番茄产量达6 700万t,位居世界首位。近年来我国番茄市场需求量还在不断攀升,但由于耕作面积有限,番茄连作障碍问题日益凸显,严重制约了番茄产业的可持续发
本文针对番茄连作障碍中的微生物作用机制以及防控研究进展进行综述,旨在基于微生物角度为防控番茄连作障碍提供理论支持,推动番茄产业的可持续发展。
土壤微生物群落结构是影响植物生长、发育和健康的主要因素,而根际土壤微生物与作物对土壤养分吸收和转化密切相关。已有的研究表明,连作导致作物根际微生物多样性下降,病原微生物丰度提
土壤细菌种群是番茄连作土壤的敏感生物学指
综上所述,番茄连作土壤中,细菌群落多样性下降,真菌丰度占比显著增加,土壤优势微生物类型由“细菌型”转变为“真菌型”。
土壤微生物多样性和丰富性对维护土壤健康和促进作物生长发挥着重要作
与单一作物连作栽培相比,作物轮作是一种用于保持土壤质量和作物生产力的一项重要农艺措
间作是指在同一生长季节在同一块土地上同时种植2种或多种作物的耕作方
嫁接是园艺作物生产中常用的一种提高作物抗逆性的繁殖方法。番茄嫁接不仅有助于提高植株抗土传病害的能
土壤消毒可消灭土壤中的病原微生物。现阶段常用的土壤消毒方法主要包括物理方法和化学方法。
Tan
木醋液是生物炭和木炭生产的二次产物,现阶段已广泛应用于土壤改良、促进作物养分吸收和植物病害控
另一方面,徐金强
番茄连作障碍问题与土壤微生物群落的变化息息相关,其作用机制十分复杂。近年来,研究发现番茄连作土壤中微生物多样性下降,土壤微生物群落结构由“细菌型”土壤转变为“真菌型”土壤,是番茄发生连作障碍重要的原因之一。
重塑连作番茄土壤微生物群落结构是有效缓解、预防番茄连作障碍的关键。轮作、间套作、嫁接、土壤消毒以及施用有机肥等措施均能够有效重塑土壤微生物群落,提升土壤中的微生物多样性。其中,将有机肥与节杆菌属(Arthrobacter)、芽孢杆菌属(Bacillus)、假单胞菌属(Pseudomonas)、土壤红色杆形菌属(Solirubrobacter)细菌以及木霉菌属(Trichoderma spp.)真菌等功能微生物结合施用,是一项极具开发前景的生物防控连作障碍发生的技术措施。但如何保障生物有机肥中有益功能微生物在不同环境中的定殖与存活,发挥出应有的功能与效果,是该项技术亟待解决的重要瓶颈问题。
参考文献 References
刘学才,陈玲,李胜奇,等.施蚯蚓粪对日光温室土壤及番茄产量与品质的影响[J].应用生态学报,2021,32(2):549-556.LIU X C,CHEN L,LI S Q,et al.Effects of vermicompost fertilization on soil,tomato yield and quality in greenhouse[J].Chinese journal of applied ecology,2021,32(2):549-556 (in Chinese with English abstract). [百度学术]
TAN G,LIU Y J,PENG S G,et al.Soil potentials to resist continuous cropping obstacle:three field cases[J/OL].Environmental research,2021,200:111319[2023-10-08].https://doi.org/10.1016/j.envres.2021.111319.。 [百度学术]
ASHWORTH A J,OWENS P R,ALLEN F L.Long-term cropping systems management influences soil strength and nutrient cycling[J/OL].Geoderma,2020,361:114062[2023-10-08].https://doi.org/10.1016/j.geoderma.2019.114062. [百度学术]
SHE S Y,NIU J J,ZHANG C,et al.Significant relationship between soil bacterial community structure and incidence of bacterial wilt disease under continuous cropping system[J].Archives of microbiology,2017,199(2):267-275. [百度学术]
杨凤军,安子靖,杨薇薇.番茄连作对日光温室土壤微生物及土壤理化性状的影响[J].中国土壤与肥料,2016(1):42-46.YANG F J,AN Z J,YANG W W.Effects of continuous cropping of greenhouse tomato on soil microbial and soil physicochemical properties[J].Soil and fertilizer sciences in China,2016(1):42-46 (in Chinese with English abstract). [百度学术]
GAO Z Y,HAN M K,HU Y Y,et al.Effects of continuous cropping of sweet potato on the fungal community structure in rhizospheric soil[J/OL].Frontiers in microbiology,2019,10:2269[2023-10-08].https://doi.org/10.3389/fmicb.2019.02269. [百度学术]
LI X Y,LEWIS E E,LIU Q Z,et al.Effects of long-term continuous cropping on soil nematode community and soil condition associated with replant problem in strawberry habitat[J/OL].Scientific reports,2016,6:30466[2023-10-08].https://doi.org/10.1038/srep30466. [百度学术]
ZHENG X F,WANG Z R,ZHU Y J,et al.Effects of a microbial restoration substrate on plant growth and rhizosphere bacterial community in a continuous tomato cropping greenhouse[J/OL].Scientific reports,2020,10(1):13729[2023-10-08].https://doi.org/10.1038/s41598-020-70737-0. [百度学术]
李坤,郭修武,孙英妮,等.葡萄连作对土壤细菌和真菌种群的影响[J].应用生态学报,2009,20(12):3109-3114.LI K,GUO X W,SUN Y N,et al.Effects of grape-replanting on soil bacterial and fungal populations[J].Chinese journal of applied ecology,2009,20(12):3109-3114 (in Chinese with English abstract). [百度学术]
刘来,黄保健,孙锦,等.大棚辣椒连作土壤微生物数量、酶活性与土壤肥力的关系[J].中国土壤与肥料,2013(2):5-10.LIU L,HUANG B J,SUN J,et al.Relationship between soil microbial quantity,enzyme activity and soil fertility in hot pepper greenhouse soils of different continuous cropping years[J].Soil and fertilizer sciences in China,2013(2):5-10 (in Chinese with English abstract). [百度学术]
李兰君,刘玳含,刘建斌,等.连作对设施番茄土壤微生物及酶活性的影响[J].江苏农业科学,2018,46(18):130-134.LI L J,LIU D H,LIU J B,et al.Effects of continuous cropping on soil microorganisms and enzyme activities of tomato in greenhouse[J].Jiangsu agricultural sciences,2018,46(18):130-134 (in Chinese). [百度学术]
MO A S,QIU Z Q,HE Q,et al.Effect of continuous monocropping of tomato on soil microorganism and microbial biomass carbon[J].Communications in soil science and plant analysis,2016,47(9):1069-1077. [百度学术]
SU L,LI H T,WANG J,et al.Composition,function and succession of bacterial communities in the tomato rhizosphere during continuous cropping[J].Biology and fertility of soils,2023,59(7):723-732. [百度学术]
LI T Z,LIU T T,ZHENG C Y,et al.Changes in soil bacterial community structure as a result of incorporation of Brassica plants compared with continuous planting eggplant and chemical disinfection in greenhouses[J/OL].PLoS One,2017,12(3):e0173923[2023-10-08].https://doi.org/10.1371/journal.pone.0173923. [百度学术]
AI C,LIANG G Q,SUN J W,et al.The alleviation of acid soil stress in rice by inorganic or organic ameliorants is associated with changes in soil enzyme activity and microbial community composition[J].Biology and fertility of soils,2015,51(4):465-477. [百度学术]
RAMPELOTTO P H,DE SIQUEIRA FERREIRA A,BARBOZA A D M,et al.Changes in diversity,abundance,and structure of soil bacterial communities in Brazilian savanna under different land use systems[J].Microbial ecology,2013,66(3):593-607. [百度学术]
FUENTES S,MÉNDEZ V,AGUILA P,et al.Bioremediation of petroleum hydrocarbons:catabolic genes,microbial communities,and applications[J].Applied microbiology and biotechnology,2014,98(11):4781-4794. [百度学术]
POLTI M A,APARICIO J D,BENIMELI C S,et al.Simultaneous bioremediation of Cr(VI) and lindane in soil by Actinobacteria[J].International biodeterioration & biodegradation,2014,88:48-55. [百度学术]
康亚龙.连作对加工番茄土壤微生物群落及多样性的影响[D].石河子:石河子大学,2015.KANG Y L.Effects of continuous cropping processing tomato on soil microbial community structure and diversity[D].Shihezi:Shihezi University,2015 (in Chinese with English abstract). [百度学术]
孙文庆,康亚龙,刘建国,等.加工番茄连作对土壤微生物群落多样性的影响[J].西北农业学报,2017,26(7):1099-1110.SUN W Q,KANG Y L,LIU J G,et al.Influence of continuous cropping of processing tomato on diversity of microflora in the rhizosphere soil[J].Acta agriculturae boreali-occidentalis sinica,2017,26(7):1099-1110 (in Chinese with English abstract). [百度学术]
KANG Y L,JING F,SUN W,et al.Soil microbial communities changed with a continuously monocropped processing tomato system[J].Acta agriculturae scandinavica,section B:soil & plant science,2018,68(2):149-160. [百度学术]
LYU J,JIN L,JIN N,et al.Effects of different vegetable rotations on fungal community structure in continuous tomato cropping matrix in greenhouse[J/OL].Frontiers in microbiology,2020,11:829[2023-10-08].https://doi.org/10.3389/fmicb.2020.00829. [百度学术]
ZHOU X G,LIU J,WU F Z.Soil microbial communities in cucumber monoculture and rotation systems and their feedback effects on cucumber seedling growth[J].Plant and soil,2017,415(1):507-520. [百度学术]
NACKE H,THÜRMER A,WOLLHERR A,et al.Pyrosequencing-based assessment of bacterial community structure along different management types in German forest and grassland soils[J/OL].PLoS One,2011,6(2):e17000[2023-10-08].https://doi.org/10.1371/journal.pone.0017000. [百度学术]
CORTEZ K J,ROILIDES E,QUIROZ-TELLES F,et al.Infections caused by Scedosporium spp[J].Clinical microbiology reviews,2008,21(1):157-197. [百度学术]
PENTON C R,GUPTA V V S R,TIEDJE J M,et al.Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing[J/OL].PLoS One,2014,9(4):e93893[2023-10-08].https://doi.org/10.1371/journal.pone.0093893. [百度学术]
TIEMANN L K,GRANDY A S,ATKINSON E E,et al.Crop rotational diversity enhances belowground communities and functions in an agroecosystem[J].Ecology letters,2015,18(8):761-771. [百度学术]
EL ZAHAR HAICHAR F,MAROL C,BERGE O,et al.Plant host habitat and root exudates shape soil bacterial community structure[J].The ISME journal,2008,2(12):1221-1230. [百度学术]
QIN S H,YEBOAH S,XU X X,et al.Analysis on fungal diversity in rhizosphere soil of continuous cropping potato subjected to different furrow-ridge mulching managements[J/OL].Frontiers in microbiology,2017,8:845[2023-10-08].https://doi.org/10.3389/fmicb.2017.00845. [百度学术]
NAVARRO-NOYA Y E,GÓMEZ-ACATA S,MONTOYA-CIRIACO N,et al.Relative impacts of tillage,residue management and crop-rotation on soil bacterial communities in a semi-arid agroecosystem[J].Soil biology and biochemistry,2013,65:86-95. [百度学术]
PERVAIZ Z H,IQBAL J,ZHANG Q M,et al.Continuous cropping alters multiple biotic and abiotic indicators of soil health[J/OL].Soil systems,2020,4(4):59[2023-10-08].https:/doi:10.3390/soilsystems4040059. [百度学术]
D’ACUNTO L,ANDRADE J F,POGGIO S L,et al.Diversifying crop rotation increased metabolic soil diversity and activity of the microbial community[J].Agriculture,ecosystems & environment,2018,257:159-164. [百度学术]
LI W H,LIU Q Z,CHEN P.Effect of long-term continuous cropping of strawberry on soil bacterial community structure and diversity[J].Journal of integrative agriculture,2018,17(11):2570-2582. [百度学术]
HUANG W,CHEN X,WANG K,et al.Comparison among the microbial communities in the lake,lake wetland,and estuary sediments of a plain river network[J/OL].Microbiology Open,2019,8(2):e00644[2023-10-08].https://doi.org/10.1002/mbo3.644. [百度学术]
LEE S,YEO I Y,SADEGHI A M,et al.Impacts of watershed characteristics and crop rotations on winter cover crop nitrate-nitrogen uptake capacity within agricultural watersheds in the Chesapeake Bay region[J/OL].PLoS One,2016,11(6):e0157637[2023-10-08].https://doi.org/10.1371/journal.pone.0157637. [百度学术]
杨尚东,李荣坦,吴俊,等.番茄连作与轮作土壤生物学特性及细菌群落结构的比较[J].生态环境学报,2016,25(1):76-83.YANG S D,LI R T,WU J,et al.Comparison of soil microbial properties and bacterial community structure in continuous cropping and rotation fields of tomatoes[J].Ecology and environmental sciences,2016,25(1):76-83 (in Chinese with English abstract). [百度学术]
TESTEN A L,CHALA A,AZEREFEGNE F,et al.First report of corky root rot of tomato caused by Pyrenochaeta lycopersici in Africa[J/OL].Plant disease,2019,103(5):1032[2023-10-08].https://apsjournals.apsnet.org/doi/10.1094/PDIS-10-18-1887-PDN. [百度学术]
邓玉清.轮作对番茄连作土壤微生物群落结构、酶活性及养分的影响[D].海口:海南大学,2018.DENG Y Q.Effects of rotation on microbial community structure,enzyme activities and nutrients in soil of continuous cropping tomato[D].Haikou:Hainan University,2018 (in Chinese with English abstract). [百度学术]
ZHOU X G,YU G B,WU F Z.Effects of intercropping cucumber with onion or garlic on soil enzyme activities,microbial communities and cucumber yield[J].European journal of soil biology,2011,47(5):279-287. [百度学术]
SINGH K,MISHRA A K,SINGH B,et al.Tillage effects on crop yield and physicochemical properties of sodic soils[J].Land degradation & development,2016,27(2):223-230. [百度学术]
LI Q S,CHEN J,WU L K,et al.Belowground interactions impact the soil bacterial community,soil fertility,and crop yield in maize/peanut intercropping systems[J/OL].International journal of molecular sciences,2018,19(2):622[2023-10-08].https://doi.org/10.3390/ijms19020622. [百度学术]
俞霞,肖世豪,李淑娟,等.禾本科-豆科间作模式中作物产量和氮素利用的研究进展[J].生态学杂志,2021,40(8):2601-2609.YU X,XIAO S H,LI S J,et al.Review on crop yield and nitrogen utilization in cereal-legume intercropping system[J].Chinese journal of ecology,2021,40(8):2601-2609 (in Chinese with English abstract). [百度学术]
LI X F,WANG Z G,BAO X G,et al.Long-term increased grain yield and soil fertility from intercropping[J].Nature sustainability,2021,4(11):943-950. [百度学术]
CHADFIELD V G A,HARTLEY S E,REDEKER K R.Associational resistance through intercropping reduces yield losses to soil-borne pests and diseases[J].The new phytologist,2022,235(6):2393-2405. [百度学术]
ROMANECKAS K,ADAMAVIČIENĖ A,ŠARAUSKIS E,et al.The impact of intercropping on soil fertility and sugar beet productivity[J/OL].Agronomy,2020,10(9):1406[2023-10-08].https://doi.org/10.3390/agronomy10091406. [百度学术]
王宇蕴,任家兵,张莹,等.小麦蚕豆间作改善蚕豆根际微生物区系与减轻蚕豆枯萎病的作用[J].土壤通报,2020,51(5):1127-1133.WANG Y Y,REN J B,ZHANG Y,et al.Effect of wheat and faba bean intercropping on improving rhizosphere microflora and reducing Fusarium wilt of faba bean[J].Chinese journal of soil science,2020,51(5):1127-1133 (in Chinese with English abstract). [百度学术]
FU X P,WU X,ZHOU X G,et al.Companion cropping with potato onion enhances the disease resistance of tomato against Verticillium dahliae[J/OL].Frontiers in plant science,2015,6:726[2023-10-08].https://doi.org/10.3389/fpls.2015.00726. [百度学术]
LI N H,GAO D M,ZHOU X G,et al.Intercropping with potato-onion enhanced the soil microbial diversity of tomato[J/OL].Microorganisms,2020,8(6):834[2023-10-08].https://doi.org/10.3390/microorganisms8060834. [百度学术]
张海春,张浩,胡晓辉.不同间作模式对温室连作番茄产量、土壤微生物和酶的影响[J].西北农业学报,2016,25(8):1218-1223.ZHANG H C,ZHANG H,HU X H.Effects of different intercropping patterns on tomato yield and soil microflora and enzymes activities in continuous cropping[J].Acta agriculturae boreali-occidentalis sinica,2016,25(8):1218-1223 (in Chinese with English abstract). [百度学术]
梅文宇,李涛,孙保娟,等.嫁接番茄砧穗互作机理研究、应用现状与展望[J].广东农业科学,2021,48(8):47-57.MEI W Y,LI T,SUN B J,et al.Research,application status and prospect on the stockscion interaction mechanism of grafted tomato[J].Guangdong agricultural sciences,2021,48(8):47-57 (in Chinese with English abstract). [百度学术]
王安全,郝德旺,蔡万聆鹤,等.嫁接对番茄抗性、产量和品质的影响研究进展[J].农业工程技术,2022,42(31):70-75.WANG A Q,HAO D W,CAI W L H,et al.Research progress on effects of grafting on resistance,yield and quality of tomato[J].Agricultural engineering technology,2022,42(31):70-75(in Chinese). [百度学术]
苏妮妮,吴浪,习鹏,等.‘砧茄一号’砧木新品种特性及其对嫁接番茄农艺性状的影响[J].西南大学学报(自然科学版),2023,45(6):97-108.SU N N,WU L,XI P,et al.Characteristics of new rootstock variety ‘Zhenqie No.1’ and its effect on agronomic traits of grafted tomatoes[J].Journal of Southwest University(natural science edition),2023,45(6):97-108 (in Chinese with English abstract). [百度学术]
刘娜,朱为民,鲁博,等.嫁接对番茄根际土壤微生态环境的影响[J].上海农业学报,2014,30(5):6-10.LIU N,ZHU W M,LU B,et al.Effects of grafting on the soil micro-ecological environment of tomato rhizosphere[J].Acta agriculturae Shanghai,2014,30(5):6-10(in Chinese with English abstract). [百度学术]
庞师婵,郭霜,任奎瑜,等.番茄/茄子嫁接对其根际土壤生物学性状及细菌群落结构的影响[J].园艺学报,2020,47(2):253-263.PANG S C,GUO S,REN K Y,et al.Impact of grafting on soil microbial properties and bacterial community structure in tomato rhizosphere[J].Acta horticulturae sinica,2020,47(2):253-263 (in Chinese with English abstract). [百度学术]
XIONG W,ZHAO Q Y,ZHAO J,et al.Different continuous cropping spans significantly affect microbial community membership and structure in a vanilla-grown soil as revealed by deep pyrosequencing[J].Microbial ecology,2015,70(1):209-218. [百度学术]
KYSELKOVÁ M,KOPECKÝ J,FRAPOLLI M,et al.Comparison of rhizobacterial community composition in soil suppressive or conducive to tobacco black root rot disease[J].The ISME journal,2009,3(10):1127-1138. [百度学术]
郝晶,周宝利,刘娜,等.嫁接茄子抗黄萎病特性与根际土壤微生物生物量和土壤酶的关系[J].沈阳农业大学学报,2009,40(2):148-151.HAO J,ZHOU B L,LIU N,et al.Relationships between grafted eggplant’s Verticillium-resistance and rhizospheric soil microbial biomass and enzyme[J].Journal of Shenyang Agricultural University,2009,40(2):148-151 (in Chinese with English abstract). [百度学术]
姜飞,刘业霞,艾希珍,等.嫁接辣椒根际土壤微生物及酶活性与根腐病抗性的关系[J].中国农业科学,2010,43(16):3367-3374.JIANG F,LIU Y X,AI X Z,et al.Study on relationship among microorganism,enzymes’ activity in rhizosphere soil and root rot resistance of grafted pepper[J].Scientia agricultura sinica,2010,43(16):3367-3374 (in Chinese with English abstract). [百度学术]
刘业霞,付玲,艾希珍,等.嫁接辣椒根系特征及根际土壤酶活性与青枯病抗性的关系[J].西北植物学报,2012,32(5):963-968.LIU Y X,FU L,AI X Z,et al.Relationship among root characteristics,activities of rhizosphere soil enzymes and bacterial wilt resistance of grafted pepper[J].Acta botanica boreali-occidentalia sinica,2012,32(5):963-968 (in Chinese with English abstract). [百度学术]
TAN X Y,LIAO H K,SHU L Z,et al.Effect of different substrates on soil microbial community structure and the mechanisms of reductive soil disinfestation[J/OL].Frontiers in microbiology,2019,10:2851[2023-10-08].https://doi.org/10.3389/fmicb.2019.02851. [百度学术]
伍朝荣.土壤厌氧消毒(ASD)改良土壤及对番茄青枯病的防控作用[D].广州:华南农业大学,2017.WU C R.Effects of anaerobic soil disinfestation on soil improvement and bacterial wilt control[D].Guangzhou:South China Agricultural University,2017 (in Chinese with English abstract). [百度学术]
秦绍龙.土壤氯氧消毒剂在设施蔬菜生产上的应用[D].杨凌:西北农林科技大学,2018.QIN S L.Application of chlorine oxygen disinfectant in facility vegetable production[D].Yangling:Northwest A & F University,2018 (in Chinese with English abstract). [百度学术]
冯明祥,王佩圣,姜瑞德,等.农药混用进行土壤消毒防治番茄根结线虫技术研究[J].农业环境科学学报,2007,26(S2):643-646.FENG M X,WANG P S,JIANG R D,et al.Application of several pesticides incorporated into soil in controlling nematodes[J].Journal of agro-environment science,2007,26(S2):643-646 (in Chinese with English abstract). [百度学术]
MIRSOLEIMANI A,NAJAFI-GHIRI M,BOOSTANI H R,et al.Relationships between soil and plant nutrients of citrus rootstocks as influenced by potassium and wood vinegar application[J].Journal of soils and sediments,2023,23(3):1439-1450. [百度学术]
ZHU K M,GU S C,LIU J H,et al.Wood vinegar as a complex growth regulator promotes the growth,yield,and quality of rapeseed[J/OL].Agronomy,2021,11(3):510[2023-10-08].https://doi.org/10.3390/agronomy11030510. [百度学术]
曾婕,海梅荣,王晓会,等.木醋液对植烟土壤微生物多样性的影响[J].土壤通报,2015,46(1):93-98.ZENG J,HAI M R,WANG X H,et al.Effects of pyroligneous acid application on microbial diversity in tobacco field[J].Chinese journal of soil science,2015,46(1):93-98 (in Chinese with English abstract). [百度学术]
肖健, 谭俊杰, 林泽毅, 等. 木醋液的抑菌活性及其对连作番茄根际土壤微环境生态的影响[J].华中农业大学学报,2024,43(1):40-51. XIAO J, TAN J J, LIN Z Y, et al. Antibacterial activity of wood vinegar and its effect on microecology in rhizospheres of tomatoes under continuous cropping system[J]. Journal of Huazhong Agricultural University,2024,43(1):40-51(in Chinese with English abstract). [百度学术]
郭伊娟.植物提取物的抑菌活性及其对番茄连作土壤微生物多样性的影响[D].南宁:广西大学,2015.GUO Y J.Inhibition of plant extracts and their effects on soil microbial diversity in tomato continuous-cropping fields[D].Nanning:Guangxi University,2015 (in Chinese with English abstract). [百度学术]
徐金强,刘素慧,刘庆涛,等.大蒜秸秆还田对温室番茄连作土壤微生物及根结线虫病的影响[J].江苏农业科学,2017,45(7):91-93.XU J Q,LIU S H,LIU Q T,et al.Effects of garlic straw returning to field on soil microbes and root-knot nematodes of tomato continuous cropping in greenhouse[J].Jiangsu agricultural sciences,2017,45(7):91-93(in Chinese). [百度学术]
徐赛,杨延杰,圣亚男,等.有机无机肥配施对日光温室番茄连作土壤微生物的影响[J].土壤通报,2022,53(4):897-906.XU S,YANG Y J,SHENG Y N,et al.Effects of combined application of organic and inorganic fertilizers on soil microorganisms in continuous cropping in solar greenhouse[J].Chinese journal of soil science,2022,53(4):897-906 (in Chinese with English abstract). [百度学术]
赵政.化肥减施与木霉生物有机肥配施对连作番茄生长和土壤微生物区系的影响[D].南京:南京农业大学,2018.ZHAO Z.Effect of Trichoderma bio-organic fertilizer with reduced chemical fertilizer application on tomato growth and on soil microbe in a monocropped system[D].Nanjing:Nanjing Agricultural University,2018 (in Chinese with English abstract). [百度学术]
孙彩霞,刘玉红,杨艳,等.不同肥料对番茄品质及连作土壤细菌群落结构的影响[J].浙江农业科学,2022,63(5):1074-1078.SUN C X,LIU Y H, SUN C X,et al.Effect of different fertilizers on tomato quality and bacterial community structure diversity of continuous cropping soil[J].Journal of Zhejiang agricultural sciences,2022,63(5):1074-1078 (in Chinese). [百度学术]
康萍芝,张丽荣,沈瑞清,等.哈茨木霉制剂对设施连作番茄根际土壤微生物的生态效应及防病作用[J].农药,2013,52(2):128-131.KANG P Z,ZHANG L R,SHEN R Q,et al.Ecological effect of Trichoderma harzianum preparations on rhizosphere soil microbes in facilities continuous cropping tomato and their disease prevention[J].Agrochemicals,2013,52(2):128-131 (in Chinese with English abstract). [百度学术]
ZHENG X F,ZHU Y J,WANG Z R,et al.Effects of a novel bio-organic fertilizer on the composition of rhizobacterial communities and bacterial wilt outbreak in a continuously mono-cropped tomato field[J/OL].Applied soil ecology,2020,156:103717[2023-10-08].https://doi.org/10.1016/j.apsoil.2020.103717. [百度学术]