Abstract:During 2021-2022, two awl nematode populations were collected from Guangdong Zhanjiang Mangrove National Nature Reserve and Guangdong Zhenhai Bay Mangrove National Wetland Park, respectively. To investigate the phylogenetic relationships among closely related species of Neodolichodorus, morphology, molecular biology and phylogenetic analyses were conducted that the two populations were identified as Neodolichodorus sinensis. The 18S rDNA and 28S rDNA D2-D3 region sequences were obtained for the