A salt-tolerant strain SF-18 with significant antibacterial activity was isolated and screened from soil samples in Aksu saline alkali land, Xinjiang. The SF-18 strain has a significant inhibitory effect on Staphylococcus aureus and Verticillium dahlia and could grow in the broth containing 18% NaCl. Using the Verticillium dahlia as an indicator strain, the SF-18 cell-free fermentation supernatant showed stable antibacterial activity within the range of -20℃-80℃, and pH 5-pH 12 environments. The strain was identified as belonging to the genus Bacillus based on the 16S rDNA gene and gyrB gene sequences. Whole gene sequence analysis showed that there were 480 genes of the strain involved in the metabolism of multiple carbon sources. The strain contained genes encoding the alimine and trehalose which are related to stress resistance. The prediction analysis of secondary metabolites showed that strain SF-18 contained gene clusters related to synthesize a various of resistant compounds, such as bacillaene, bacillibactin, mersacidin, fengycin, zwittermicin, as well as genes related to glucanase and chitinase that can hydrolyze the cell wall of pathogen. Therefore, it is speculated that strain SF-18 can produce antibacterial secondary metabolites and related degrading enzymes to prevent and control plant disease, showing good application prospects in agricultural biological control.