猪精液冻融技术及其影响因素研究进展
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华中农业大学动科学院

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国家自然科学基金项目(31941006)


Research progress on porcine semen cryopreservation technology and its influencing factors
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National Natural Science Foundation of China (31941006)

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    摘要:

    养猪业是我国畜牧业产业的重要组成。国内生猪养殖主要采用自繁自养模式,配种所使用的精液多采用鲜精或常温稀释保存精液,而精液冻融技术可实现猪精液的长期保存,但与牛、小鼠等物种相比较,猪精液冻融技术还有待于进一步优化改善。本文主要综述了猪精液冻融技术发展概况、主要技术流程,影响猪精液冻融后精子活力的关键因素,包括精液的采集、冷冻前降温平衡、冷冻保护剂添加和冷冻方法等,以及精子冻融过程中的理化性质变化,包括物理结构损伤、功能性损伤、精子抗冻机制等,最后总结了能够提高猪精液冻融效果的新方法,包括纳米技术处理精液、精子耐冻生物标志物的研究等,旨在为进一步改进猪精液冷冻技术并实现在生产上大规模应用提供理论参考。

    Abstract:

    Pig production is an important component of animal husbandry industry in China. Domestic pig production mainly uses self-breeding mode. The semen used in pig reproduction mostly uses fresh semen or diluted semen stored in room temperature, and semen cryopreservation technology can make the long-term preservation of pig semen. Compared with cattle, mice and other species, the cryopreservation technology of pig semen needs to be further optimized and improved. This review summarizes the development of frozen pig semen, the main technical process, the key factors affecting sperm motility after freezing and thawing, including semen collection, pre-cooling balance before freezing, addition of cryoprotectant agents, and cryopreservation methods. It also covers the physicochemical changes during the sperm cryopreservation process, such as physical structural damage, functional damage, and sperm anti-freezing mechanisms. Finally, new methods to improve the cryopreservation efficiency of pig sperm are summarized, including nanotechnology treatment of semen and the study of cryopreservation biomarkers for sperm,in order to provide theoretical reference for perfecting porcine frozen semen technology and accelerating its application in pig production.

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  • 收稿日期:2022-06-13
  • 最后修改日期:2023-11-11
  • 录用日期:2023-12-19
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