Black soldier flies（Hermetia illucens L.） were reared with a holidic medium to study the characteristics of nutritional requirements and key nutrient factors for the black soldier fly.The growth and development of black soldier flies were evaluated by controlling nitrogen sources，carbon sources，the ratio of carbon-to-nitrogen and ratio of larvae-to-dry feed.The results showed that the black soldier fly reared on the holidic medium had better growth performance with the larval survival rate of （84.38 ± 1.18）%，the larval duration of （29.04±0.43） d，the peak individual fresh mass of （119.03±1.82） mg，the pupation rate of （60.59±1.50）%，the pupal duration of （14.70±0.16） d，and the emergence rate of （80.20±3.65）%.The performance of holidic medium was better than that of Drosophila medium，although the enriched medium was superior in certain aspects.The results of comprehensively analyzing the fresh weight of individual larvae，the rate of feed reduction，and the bioconversion rate of larvae showed that amino acids were the optimal nitrogen source，glucose and starch were the optimal carbon sources，the most suitable ratio of carbon-to-nitrogen was 15，and the optimal ratio of larvae-to-dry feed was 1∶0.15.It is indicated that the holidic medium developed in this article can enable black soldier flies to complete its life cycle，achieving better growth performance under the conditions of using amino acids，glucose，starch as the optimal carbon sources，with the suitable ratio of carbon-to-nitrogen of 15 and the optimal ratio of larvae-to-dry feed of 1∶0.15.