The efficient and stable genetic transformation system of plants is an important technical support for the analysis of gene function and molecular breeding. However， the imperfect tissue culture system seriously hinders its application in many plants， especially in crops. In recent years， many efficient genetic transformation systems of plants with non-tissue culture have been established to solve the problems mentioned above. This article focuses on the genetic transformation system of plants with non-tissue culture mediated by Agrobacterium tumefaciens in terms of the current status of application， factors affecting transformation efficiency， and applications of gene editing with flower organ infection， cut-dip-budding （CDB） delivery， regenerative activity dependent in plant injection delivery （RAPID）， seed inoculation， development regulatory （DR） assisted transformation， and virus delivery in plants. The application of pollen tube channel method， particle bombardment method and nano delivery method in plants independent of Agrobacterium tumefaciens was summarized. It will provide reference for establishing efficient， simple and genotype-independent genetic transformation system with non-tissue culture in more species and assisting the studies on plant functional genes and the practices of molecular breeding.