1.华中农业大学植物科学技术学院/湖北省作物病害检测和安全控制重点实验室，武汉 430070;2.国家柑橘保鲜技术研发专业中心，武汉 430070;3.宜昌市夷陵区农业技术服务中心，宜昌 443100
张家宁，E-mail：zhangjianing199904@163.com
付艳苹，E-mail： yanpingfu@mail.hzau.edu.cn
S663.1
国家重点研发计划项目（2021YFD1600103）；湖北省重点研发计划项目（2021BBA095）；现代农业产业技术体系建设专项（CARS-26）
- ZHANG Jianing 1,2
- LONG Fuhan 1,2
- YANG Zhonghua 1
- LI Yarong 1
- XIE Heping 3
- LIN Yang 1
- ZHENG Wenyan 3
- YU Xiao 1
- FU Yanping 1,2
1.College of Plant Science ＆ Technology/Hubei Province Key Laboratory of Plant Pathology， Huazhong Agricultural University，Wuhan 430070， China;2.National R & D Center for Citrus Postharvest Technology，Wuhan 430070， China;3.Yichang Yiling District Agricultural Technology Service Center， Yichang 443100， China
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为了科学规范咪鲜胺和抑霉唑在柑橘果实保鲜处理中的使用，基于HPLC建立一种同时检测较高质量浓度条件下咪鲜胺和抑霉唑的方法。结果显示，在5~300 μg/mL范围内，咪鲜胺和抑霉唑线性关系良好，该方法灵敏度和准确度高。温州蜜柑采后分选线上，室温条件下咪鲜胺稳定，抑霉唑降解率高，工作6 h处理温州蜜柑15 t，咪鲜胺和抑霉唑质量浓度分别降低98.6、290.3 μg/mL。结果表明，该方法可以应用于柑橘采后处理过程中咪鲜胺和抑霉唑的质量浓度检测，有利于提高杀菌剂使用效率及降低生产成本。
A method for simultaneously detecting the concentrations of Prochloraz and Imazalil at relatively high concentrations was established with high performance liquid chromatography （HPLC） to scientifically standardize the use of Prochloraz and Imazalil in the preservation of citrus fruits.The results showed that the linear relationship between Prochloraz and Imazalil was good within the range of 5-300 μg/mL， indicating that this method has high sensitivity and accuracy.Prochloraz was stable at room temperature and Imazalil had a high rate of degradation in the post-harvest sorting line of Satsuma mandarins.The concentration of Prochloraz and Imazalil after 6 hours of processing 15 tons of Satsuma mandarins decreased by 98.6 μg/mL and 290.3 μg/mL， respectively.It is indicated that this method can be applied to detect the concentration of Prochloraz and Imazalil during the post-harvest processing of citrus fruits， which is beneficial for improving the efficiency of using fungicide and reducing costs of production.
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张家宁,龙复涵,杨中华,李亚荣,谢合平,林杨,郑文艳,于晓,付艳苹.柑橘采后分选中咪鲜胺和抑霉唑浓度检测[J].华中农业大学学报,2025,44(2):221-227
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- 收稿日期:2024-10-28
- 在线发布日期: 2025-04-02
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