Pinellia ternata diseases pose a significant obstacle to the industrial development of P. ternata.To screen for superior strains of beneficial microorganisms that promote growth and enhance resistance to P. ternata diseases，strains were isolated and screened from the rhizosphere soil of P. ternata.Nine strains exhibiting antagonistic effects against various pathogens of P. ternata，including Fusarium oxysporum，Aspergillus，Fusarium，Phytophthora capsici and Fusarium solani，were successfully isolated.These strains were identified，and their antimicrobial activities were assessed.Subsequently，the strains were formulated into microbial fertilizers，and their effects on promoting growth promotion and controlling disease in P. ternata were evaluated.The results showed that strain 21-2 exhibited the strongest antimicrobial activity，and identification confirmed that the strain was Paenibacillus polymyxa.Strain 21-2 demonstrated notable inhibitory effects against five common pathogens of P. ternata and significantly promoted the germination of P. ternata seeds.The bacterial suspension of strain 21-2 exhibited a disease control efficacy of 45.83% against diseases affecting P. ternata and significantly enhanced the plant’s height，fresh weight，bulb weight，and chlorophyll content .The bio-organic fertilizer，which consists of P. polymyxa 21-2，straw-derived biochar，and organic fertilizer from chicken manure，exhibited a disease control efficacy of 61.90% against diseases affecting P. ternata.The results indicated that the P. polymyxa strain 21-2 could be effectively utilized for controlling diseases in P. ternata，promoting its growth，and demonstrating significant potential for further development and application.