首页 > 过刊浏览>2024年第43卷第6期 >261-269. DOI:10.13300/j.cnki.hnlkxb.2024.06.025
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万寿菊花瓣叶黄素含量与颜色系数的相关性分析
作者:
作者单位:

1.华中农业大学园艺林学学院/果蔬园艺作物种质创新与利用全国重点实验室，武汉 430070;2.云南立达尔生物科技有限公司，文山 663100;3.广州立达尔生物科技股份有限公司，广州 510663;4.北京市园林绿化科学研究院， 北京 100102

作者简介:

吕桂芳，E-mail：1069905818@qq.com

通讯作者:

何燕红，E-mail：hyh2010@mail.hzau.edu.cn
臧运平，E-mail：1072580426@qq.com

中图分类号:

S682.1+1

基金项目:

中央引导地方科技发展资金项目（YDZX20214400003224）


Correlation analysis of lutein content and color coefficient in petals of marigold （Tagetes erecta）
Author:
Affiliation:

1.College of Horticulture and Forestry Sciences/National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops，Huazhong Agricultural University，Wuhan 430070，China;2.Yunnan Lidar Biological Technology Co.， Ltd.，Wenshan663100，China;3.Guangzhou Lidar Biotechnology Co.， Ltd.，Guangzhou 510663，China;4.Beijing Institute of Landscape Architecture，Beijing 100102，China

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    摘要:

    为建立一种快速测定万寿菊（Tagetes erecta）花瓣叶黄素含量的方法，利用色差仪测得万寿菊花瓣的亮度值L*、红绿值a*、黄蓝值b*，并计算a*/b*、（a*/b*）2、色度值、色调值和色光值等颜色系数，同时采用紫外-可见分光光度法结合HPLC法测定花瓣叶黄素含量，分析叶黄素含量与这些颜色系数的相关性，建立回归方程并进行验证。结果显示，万寿菊花瓣叶黄素含量与a*、a*/b*、（a*/b*）2、色调值和色光值极显著相关；用三次函数模型构建的万寿菊叶黄素含量与a*值之间的回归方程拟合程度最高，且叶黄素含量的预测值与实测值之间差异不显著。结果表明，基于a*值构建的三次函数模型可以快速、无损预测万寿菊花瓣叶黄素含量。

    Abstract:

    The colorimeter was used to measure the values of L* （brightness），a* （redness-greenness） and b* （yellowness-blueness） in petals of marigold （Tagetes erecta） to establish a rapid method for determining the lutein content in petals of marigold.The color coefficients including a*/b*，（a*/b*）2，chroma value，hue value and shade value were calculated.UV-spectrophotometry combined with HPLC method was used to determine the lutein content in petals.The correlation between the lutein content and the color coefficients was analyzed.The regression equation was established and confirmed.The results showed that the lutein content in petals of marigold was significantly correlated with a*、a*/b*、（a*/b*）2，hue value and shade value.The regression equation between the lutein content and a* value constructed by cubic function model had the highest degree of fitness，and there was no significant difference between the predicted value and the measured value of the lutein content.It is indicated that the cubic function model based on a* value can quickly and non-destructively predict the content of lutein petals of marigold.

    图1 不同万寿菊基因型的花色Fig.1 Flower colors of different marigold genotypes
    图2 不同万寿菊基因型花瓣的叶黄素含量Fig.2 Lutein content of petals of different marigold genotypes
    图3 4个回归模型对应的叶黄素含量预测值与4个万寿菊基因型的实测值对比Fig.3 Comparison of the predicted lutein content of the four regression models with the measured values of the four marigold genotypes
    表 1 不同万寿菊基因型的基本信息Table 1 Basic information about different marigold genotypes
    表 2 不同万寿菊基因型花瓣的颜色系数Table 2 Color coefficient of petals from different marigold genotypes
    表 3 万寿菊花瓣叶黄素含量与颜色系数的相关性Table 3 Correlation between lutein content and color coefficient of marigold petals
    表 4 万寿菊花瓣颜色系数与叶黄素含量的回归方程Table 4 Regression equations of color coefficient and lutein content of marigold petals
    表 5 叶黄素含量实测值与预测值之间的配对t检验结果Table 5 Paired t-test results between the measured and predicted values of lutein content
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吕桂芳,臧运平,彭逸,陶正国,炎天埸,张华丽,刘建,何燕红.万寿菊花瓣叶黄素含量与颜色系数的相关性分析[J].华中农业大学学报,2024,43(6):261-269

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