1.果蔬园艺作物种质创新与利用全国重点实验室/华中农业大学园艺林学学院,武汉 430070;2.云南省农业科学院热带亚热带经济作物研究所,保山 678000;3.云南省热带作物科学研究所,景洪 666100
刘承浪，E-mail：448827890@qq.com
邓秀新，E-mail：xxdeng@mail.hzau.edu.cn
S666
云南省科技厅重大科技专项（202102AE090054）；国家现代农业（柑橘）产业技术体系项目（CARS-26）
- LIU Chenglang 1,2
LIU Chenglang
National Key Laboratory for Germplasm Innovation &Utilization of Horticultural Crops/ College of Horticulture and Forestry Sciences，Huazhong Agricultural University， Wuhan 430070，China;Institute of Tropical and Subtropical Cash Crops，Yunnan Academy of Agricultural Sciences， Baoshan 678000，China
在期刊界中查找
在百度中查找
在本站中查找 - HUANG Wenkai 1
- YAN Suyun 2
- ZHOU Xianyan 2
- DONG Meichao 2
- XU Xiangzeng 3
- GAO Shide 3
- GAO Junyan 2
- CHAI Lijun 1
- DENG Xiuxin 1
1.National Key Laboratory for Germplasm Innovation &Utilization of Horticultural Crops/ College of Horticulture and Forestry Sciences，Huazhong Agricultural University， Wuhan 430070，China;2.Institute of Tropical and Subtropical Cash Crops，Yunnan Academy of Agricultural Sciences， Baoshan 678000，China;3.Yunnan Institute of Tropical Crops，Jinghong 666100，China
- 摘要 |
- 图/表 |
- 访问统计 |
- 参考文献 |
- 相似文献 [20] | | |
- 文章评论
为明确‘东试早柚’果实无核成因，以‘东试早柚’及其杂交群体为试材，利用醋酸洋红和苯胺蓝染色法、杂交授粉及分子标记等手段探究‘东试早柚’无核原因。结果显示：‘东试早柚’的花结构完整，花粉活力及萌发率分别为94.80%和86.20%；‘东试早柚’自交表现为不亲和，而与‘沙田柚’杂交亲和；利用S5、S6基因型分子标记从‘沙田柚’ב东试早柚’的175株杂交子代中共鉴定出F1代杂种164株，排除了‘东试早柚’雄性不育的可能；连续2 a田间试验观察显示，‘东试早柚’刺激性单性结实和天然单性结实的平均座果率分别为13.04%和25.56%。结果表明，‘东试早柚’自交不亲和并具有一定的单性结实能力是导致其无核的主要成因。
‘Dongshi Zaoyou’ pummelo and its hybrid offsprings，methods including magenta acetate staining and aniline blue staining，cross-pollination，and molecular markers were used to study the mechanism of seedlessness in ‘Dongshi Zaoyou’ pummelo.The results showed that the floral structure of ‘Dongshi Zaoyou’ pummelo was intact，with pollen vitality and germination rates of 94.80% and 86.20%，respectively.The self-pollination of ‘Dongshi Zaoyou’ pummelo showed incompatibility，while cross-pollination with ‘Shatianyou’ pummelo showed compatibility.A total of 164 F1 hybrids were identified from 175 hybrid offsprings of ‘Shatianyou’ pummelo × ‘Dongshi Zaoyou’ pummelo with S5/S6 molecular marker genotyping，ruling out the possibility of male sterility in ‘Dongshi Zaoyou’ pummelo.The results of observation for two consecutive years showed that the mean fruit setting rates of stimulating parthenocarpy and natural parthenocarpy in ‘Dongshi Zaoyou’ pummelo was 13.04% and 25.56%，respectively.It is indicated that the main reason for the seedless development of ‘Dongshi Zaoyou’ pummelo is its self-incompatibility and certain ability to produce parthenocarpy.
引用本文
刘承浪,黄文铠,闫素云,周先艳,董美超,徐祥增,高世德,高俊燕,柴利军,邓秀新.‘东试早柚’果实无核成因探究[J].华中农业大学学报,2024,43(5):91-97
复制
文章指标
- 点击次数:67
- 下载次数: 589
- HTML阅读次数: 39
历史
- 收稿日期:2024-01-24
- 在线发布日期: 2024-10-08
文章二维码
