广西壮族自治区农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室/ 农业农村部广西甘蔗生物技术与遗传改良重点实验室，南宁 530007
张保青，E-mail：zbqsxau@126.com
高轶静，E-mail：yijinggao@aliyun.com
S566.1
广西科技重大专项（桂科AA22117002-1）；广西自然科学基金项目（2023GXNSFAA026354）；国家糖料产业技术体系项目（CARS-17）；中国科学院华南植物园重点支持项目（E36101）；广西农业科学院基本科研业务专项（桂农科2021YT004；桂农科2024YP028）
Institute of Sugarcane， Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Ministry of Agriculture and Rural Affairs Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi)， Nanning 530007， China
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为发掘甘蔗育种优异野生基因资源，以来自广西的333份割手密为材料，应用12对SSR引物的分子标记数据和28个表型性状资料构建广西割手密核心种质，并进行关联分析。关联分析结果显示，广西割手密茎径、节间长度、曝光前颜色、脱叶性和毛群共5个表型性状与8个位点显著相关；茎径与叶长、叶宽、节间长度、节数和锤度5个性状均表现出极显著相关；株高、茎径、节间长度、节数4个表型性状之间呈现显著或极显著的正相关；锤度与茎径和节间长度均表现为极显著的负相关。核心种质抽样按照总资源比例的0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9进行梯度筛选，当抽样比例达到30%以上时，即可包含100%等位基因覆盖度。遗传多样性评价和主成分分析结果显示，构建割手密核心种质所筛选材料具有丰富的遗传多样性，且基于核心种质绘制的主成分图与原始种质的分布图趋势相吻合。结果表明，根据30%的抽样比例筛选出99个割手密样本构建核心种质，遗传多样性评价和主成分分析所构建的核心种质具有较好的代表性。
333 accessions of Saccharum spontaneum L. from Guangxi were used to mine the excellent wild genetic resources for sugarcane breeding. The data from 12 pairs of SSR primers and 28 phenotypic traits were used to construct the core germplasm and conduct association analysis of Saccharum spontaneum L. in Guangxi. The results of association analysis showed that 5 phenotypic traits including the stem diameter， the internode length， the color before exposure， defoliation and hairiness were significantly correlated with 8 SSR loci. The diameter of stem was significantly correlated with leaf length， leaf width， internode length， number of nodes and brix. There was a significant to extremely significant positive correlation between the 4 phenotypic traits including the plant height， stem diameter， internode length， and number of nodes. The brix was negatively correlated with stem diameter and internode length. Core germplasm sampling was subjected to gradient screening based on the proportion of total resources at 0.1， 0.2， 0.3， 0.4， 0.5， 0.6， 0.7， 0.8， and 0.9. When the sampling ratio reached 30% or more， it covered 100% allele. The results of evaluating genetic diversity and principal component analysis showed that the screened materials for constructing the core germplasm of Saccharum spontaneum L. had rich genetic diversity， and the principal component map drawn based on the core germplasm was consistent with the distribution map of the original germplasm. It is indicated that the core germplasm of Saccharum spontaneum L. constructed with 99 selected samples based on a sampling ratio of 30% has good representativeness by evaluating genetic diversity and principal component analysis.
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张保青,黄玉新,周珊,段维兴,杨翠芳,张革民,陆衫羽,高轶静.广西割手密核心种质构建与关联分析[J].华中农业大学学报,2024,43(5):75-81
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- 收稿日期:2023-11-08
- 在线发布日期: 2024-10-08
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