首页 > 过刊浏览>2024年第43卷第3期 >148-157. DOI:10.13300/j.cnki.hnlkxb.2024.03.015
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贵州荞麦重要种质的遗传多样性分析及分子身份证构建
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作者单位:

贵州大学生命科学学院/农业生物工程研究院/山地植物资源保护与种质创新教育部重点实验室，贵阳 550025

作者简介:

冷宇鑫， E-mail： 1960034828@qq.com

通讯作者:

文晓鹏，E-mail：xpwensc@hotmail.com

中图分类号:

S517.102.4

基金项目:

贵州省科技厅中央引导地方发展资金项目（黔科合中引地［2023］009）；贵州省农业农村厅种业发展项目（黔农计财［2022］9号）冷宇鑫，E-mail：1960034828@qq.com


Genetic diversity and ID construction of important buckwheat germplasm in Guizhou Province based on SSR markers
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Affiliation:

College of Life Sciences/Institute of Agro-bioengineering/Ministry of Education Key Laboratory of Plant Resource Conservation and Germplasm lnnovation in Mountainous Region， Guizhou University， Guiyang 550025， China

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    摘要:

    为精准鉴定贵州荞麦种质资源，采用SSR分子标记对60份荞麦种质进行遗传多样性分析，构建DNA分子身份证数据库。结果显示，从100对SSR引物中筛选出16对稳定性好、多态性丰富的引物，在60份供试种质中共扩增出174个多态性条带；Shannon’s信息指数、Nei’s多样性指数、多态信息指数均值分别为0.337、0.206、0.693，引物的多态性较好，能有效揭示60份荞麦种质的遗传多样性；在Dice遗传相似系数为0.374时，所有供试材料可聚为A、B、C 三组；当Dice遗传相似系数为0.484时，将苦荞组（A组）更细分为A1、A2两个小组。采用毛细管电泳及8%的聚丙烯酰胺凝胶（PAGE）电泳对SSR标记扩增产物进行双验证，2种方法的聚类结果一致。结果表明，本研究开发的高效性SSR分子标记能够有效地鉴定贵州荞麦重要种质的遗传多样性且用于构建分子身份证。

    Abstract:

    SSR markers were used to analyze the genetic diversity and construct a DNA molecular ID database of 60 buckwheat germplasms in order to accurately identify the buckwheat germplasm resources in Guizhou province. The results showed that 16 pairs of stable and polymorphic primers were screened from 100 pairs of SSR primers. A total of 174 polymorphic bands were amplified from 60 germplasm studied. The mean value of Shannon’s information index， Nei’s diversity index， and polymorphic information index was 0.337， 0.206， and 0.693， respectively. It is indicated that the polymorphism of primers is good， which can effectively identify the genetic diversity of 60 buckwheat germplasms. When the Dice genetic similarity coefficient was 0.374， all materials tested were clustered into three groups including A， B， and C. When the Dice genetic similarity coefficient was 0.484， the Tartary buckwheat group （Group A） was further divided into two subgroups including A1 and A2. The results of clustering SSR amplification products validated by capillary electrophoresis and 8 polyacrylamide gel （PAGE） electrophoresis were consistent. It is indicated that the efficient SSR molecular markers developed can effectively identify the genetic diversity and can be used to construct DNA molecular ID cards of important buckwheat germplasm in Guizhou province.

    表 1 供试荞麦种质信息Table 1 The information of the tested buckwheat germplasms
    表 2 16对SSR引物信息Table 2 The information of 16 pairs of SSR primers
    表 3 SSR引物扩增结果统计Table 3 Statistics of SSR primers amplification results
    图1 部分SSR引物的2%琼脂糖电泳图Fig.1 The profiles amplified by several primers using 2% agarose gel electrophoresis
    图2 SSR98引物在5个荞麦样本中的毛细管电泳图Fig.2 Capillary electrophoresis of SSR98 primer in five samples
    图3 基于毛细管电泳的荞麦种质SSR标记UPGMA聚类图Fig.3 UPGMA dendrograms of 60 buckwheat germplasms as revealed by SSR markers
    图4 引物SSR-13扩增荞麦种质的毛细管电泳图Fig.4 The capillary electrophoretic profiles of buckwheat germplasms as revealed by primer pair SSR-13
    图5 引物SSR-13扩增的60份荞麦聚丙烯酰胺凝胶电泳图Fig.5 The polyacrylamide gel electrophoretic profiles of buckwheat germplasms
    图6 基于聚丙烯酰胺凝胶电泳的荞麦种质SSR标记UPGMA聚类图Fig.6 UPGMA dendrograms of 60 buckwheat germplasms as revealed by SSR markers
    图7 荞麦种质贵米苦荞55号和九江苦荞分子身份证Fig.7 Molecular IDs of buckwheat cultivars Guimikuqiao 55 and Jiujiangkuqiao
    表 4 SSR引物鉴别供试种质情况Table 4 The number of germplasms that can be identified by SSR primers
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冷宇鑫,闵义,付阳云,周奎,何颖,文晓鹏.贵州荞麦重要种质的遗传多样性分析及分子身份证构建[J].华中农业大学学报,2024,43(3):148-157

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  • 收稿日期:2023-09-18
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