It is of great theoretical significance to study the changing characteristics of net carbon emissions and the capacity of regional carbon neutrality for accelerating the transformation of social and economic development and realizing the goal of carbon neutrality. In this article， the carbon emissions， carbon sinks and net carbon emissions in Hubei Province were evaluated with the methods given in IPCC Guidelines for National Greenhouse Gas Inventories and Guidelines for the Preparation of Provincial Greenhouse Gas Inventories， carbon density method and other methods. The changing trend of net carbon emissions was studied to evaluate the capacity of regional carbon neutrality in Hubei Province. The results showed that the total amount of regional carbon sinks in Hubei Province from 2000 to 2020 was 264.178 million tons， showing a significant upward trend over the years， with an increase rate of about 135.21% and an average annual increase rate of about 6.76%. The total carbon emissions in Hubei Province from 2000 to 2020 was 910.331 million tons. The changing trend of total carbon emissions over the years was divided into two stages including the total carbon emissions in Hubei Province from 2000 to 2010 and from 2011 to 2020 showing a trend of significant increase and a trend of fluctuating downward， respectively. The net carbon emissions in Hubei Province from 2000 to 2010 had an upward trend， and the Z_M-K statistic passed the significance test with statistical significance. The net carbon emissions in Hubei Province from 2011 to 2020 had a downward trend， but the Z_M-K statistic failed the significance test， indicating that the net carbon emissions in Hubei Province had a downward trend in the second stage， but this trend was not statistically significant. It is indicated that there is still a huge gap between the carbon sink and carbon emissions of the ecosystem in Hubei Province， and there is still some uncertainty in the changes of net carbon emissions. Carbon reduction should be continued. The carbon sink function of the ecosystem should be optimized， and carbon capture technology and related industries vigorously developed.