Ecological space is an important vehicle for maintaining regional carbon balance and securing carbon storage， and the redline area of ecological protection is an important part of ecological space. This article aimed at studying the spatial and temporal response of the carbon storage of the ecological space under the constraints of the ecological bottom line. The ArcGIS platform was used to integrate the PLUS model and the InVEST-Carbon model to predict and simulate the changes in carbon storage of ecological space and its spatial characteristics in Xiangyang City， Hubei Province， China， from 2000 to 2030. The results showed that the area of ecological space in Xiangyang City decreased between 2000 and 2020. It is predicted that the degradation rate of ecological space will decrease by 2030 under the constraints of the ecological bottom line. By 2030， the carbon storage in Xiangyang City will undergo a corresponding transformation due to changes in ecological space. The maximum carbon storage per grid unit is about 18.47 kg， mainly distributed in the forest land in the west， northeast， and southeast. The hotspot analysis presents a pattern of “concentrated hotspot and scattered cold spots”. The constraint of ecological bottom line helps to optimize the pattern of carbon hotspot in the ecological space of Xiangyang City. It will provide scientific guidance for implementing “carbon peaking and carbon neutrality” strategies and promoting low-carbon planning in similar regions.