首页 > 过刊浏览>2024年第43卷第2期 >188-196. DOI:10.13300/j.cnki.hnlkxb.2024.02.021
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酵母铬和二氢吡啶对热应激围产期奶牛生理生化指标及HSP70 mRNA表达量的影响
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作者:
作者单位:

1.华中农业大学动物科学技术学院，武汉 430070;2.武汉城市职业学院财经学院，武汉 430064

作者简介:

张李文，E-mail：1594848149@qq.com

通讯作者:

齐智利，E-mail：zhiliqi@mail.hzau.edu.cn

中图分类号:

S858.23

基金项目:

国家重点研发计划项目（2018YFD0501605；2016YFD0500507）；广东省畜禽育种与营养研究重点实验室开放课题（2021SZ04）


Effects of dietary supplementation of yeast chromium and dihydropyridine on physiological and biochemical indices and HSP70 mRNA expression of periparturient dairy cows under heat stress
Author:
Affiliation:

1.College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2.Institute of Finance and Economics, Wuhan City Polytechnic, Wuhan 430064, China

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    摘要:

    为确定酵母铬和二氢吡啶是否能缓解围产期奶牛热应激，将24头体质量和胎次相近的围产期荷斯坦奶牛随机分为3组，饲养于环境温湿指数（THI）高于68的热应激条件下，对照组饲喂基础日粮，酵母铬组和二氢吡啶组饲喂基础日粮的同时饲喂8 g/（头·d）铬含量为1‰的酵母铬和3 g/（头·d）的二氢吡啶，试验周期为40 d，观测酵母铬和二氢吡啶对围产期奶牛热应激反应的影响。结果显示：① 酵母铬组和二氢吡啶组奶牛与对照组奶牛在呼吸频率上无显著差异（P>0.05）。② 各组奶牛血清中血糖和胆固醇含量在产前第7天与产后第3天均无显著差异。二氢吡啶组奶牛产前第7天血清中甘油三酯含量显著降低（P<0.05）。③ 与对照组比较，酵母铬组和二氢吡啶组产前、产后血清中Na+、K+、Cl-的浓度均无显著差异（P>0.05），各组在产前、产后血清中碱性磷酸酶和肌酸激酶的含量也均未出现显著变化（P>0.05）。④ 酵母铬组、二氢吡啶组与对照组奶牛血清中热休克蛋白70（heat shock protein 70，HSP70）基因mRNA表达量没有明显差异（P>0.05）。以上结果表明，热应激条件下，在围产期奶牛日粮中添加8 g/（头·d）铬含量为1‰的酵母铬和3 g/（头·d）二氢吡啶对血液生化指标和HSP70 mRNA表达量均无显著影响，说明日粮中添加8 g/（头·d）铬含量为1‰的酵母铬和3 g/（头·d）二氢吡啶对缓解围产期荷斯坦奶牛热应激无明显效果。

    Abstract:

    In order to investigate the effects of dietary supplementation of yeast chromium and dihydropyridine on physiological and biochemical indices and HSP70 mRNA expression of periparturient dairy cows under heat stress， a total of 24 periparturient Holstein‐Friesian cows with similar body weights and parity were randomly assigned to three groups. Cows were housed at high ambient temperature-humidity index（above 68）for 40 d， and the effects of yeast chromium and dihydropyridine on the heat stress response of perinatal dairy cows were observed during the experimental period. The control group was fed with basal diet， the chromium yeast group was fed with basal diet plus chromium 1‰ and the amount of chromium yeast was 8 g/d， and the dihydropyridine group was fed with basal diet plus dihydropyridine and the amount of chromium yeast was 3 g/d. The results showed as follows： ① Compared with the control group， there was no significant difference in respiratory rate of periparturient dairy cows in chromium yeast group and dihydropyridine group （P>0.05）. ② Compared with the control group， there was no significant difference in serum blood glucose and cholesterol in yeast chromium group and dihydropyridine group on the 7th day before delivery and the 3rd day after delivery （P>0.05）. Compared with the control group， the content of serum triglyceride decreased significantly in dihydropyridine group on the 7th day before delivery （P<0.05）. ③ Compared with the control group， there was no significant differences in serum Na+， K+ and Cl- concentrations in chromium yeast group and dihydropyridine group before and after delivery （P>0.05）， and the concentrations of serum alkaline phosphatase and creatine kinase did not change significantly in the two experimental groups before and after delivery （P>0.05）. ④ There was no significant difference in the expression of heat shock protein 70 mRNA in serum between chromium yeast group， dihydropyridine group and control group （P>0.05）. In conclusion， dietary supplementation of 8 g/d yeast chromium with 1‰ chromium and 3 g/d dihydropyridine had no significant effects on physiological and biochemical indices and HSP70 mRNA expression of periparturient dairy cows under heat stress. These results indicated that supplementation of 8 g/d yeast chromium with 1‰ chromium and 3 g/d dihydropyridine had no significant effect on relieving heat stress of perinatal Holstein dairy cows.

    表 2 酵母铬和二氢吡啶处理下热应激围产期奶牛呼吸频率Table 2 Respiratory rate of perinatal dairy cows under heat stress treated with yeast chromium and dihydropyridine
    表 3 酵母铬和二氢吡啶处理下热应激围产期奶牛血清生化代谢物质浓度变化Table 3 Changes of serum biochemical metabolites concentration in perinatal dairy cows under heat stress treated with yeast chromium and dihydropyridine
    表 4 酵母铬和二氢吡啶处理下热应激围产期奶牛血液电解离子浓度变化Table 4 Changes of electrolytic ion concentration in blood of perinatal dairy cows under heat stress treated with yeast chromium and dihydropyridine
    图1 牛舍每日07：00、13：00和17：00的温湿指数Fig.1 Temperature humidity index of cowshed at 07：00， 13：00 and 17：00 every day
    图2 酵母铬或二氢吡啶处理下热应激围产期奶牛血液HSP70 mRNA表达量Fig.2 Expression of HSP70 mRNA in blood of perinatal cows treated with yeast chromium or dihydropyridine under heat stress
    表 5 酵母铬和二氢吡啶处理下热应激奶牛碱性磷酸酶和肌酸激酶活性的变化Table 5 Changes of alkaline phosphatase and creatine kinase in heat-stressed dairy cows treated with yeast chromium and dihydropyridine
    表 1 奶牛产前和产后日粮的组成和营养水平Table 1 The composition and nutrient levels of the experimental diets
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张李文,陈慧君,王超,张绍波,王盛琪,张博,敖英男,齐智利.酵母铬和二氢吡啶对热应激围产期奶牛生理生化指标及HSP70 mRNA表达量的影响[J].华中农业大学学报,2024,43(2):188-196

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