果蔬园艺作物种质创新与利用全国重点实验室/华中农业大学园艺林学学院，武汉 430070
李蕊蕊，E-mail：1552714384@qq.com
罗靖，E-mail：ljcau@mail.hzau.edu.cn
S682.2
国家重点研发计划项目（2018YFD1000407）；中央高校基础研究基金项目（2662015BQ008）；国家自然科学基金项目（31401911；32172611）
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ College of Horticulture and Forestry Sciences， Huazhong Agricultural University， Wuhan 430070， China
- 摘要 |
- 图/表 |
- 访问统计 |
- 参考文献 |
- 相似文献 |
- 引证文献 | |
- 文章评论
为明确百合（Lilium spp.）花朵衰老发生的分子机制，在东方百合‘西伯利亚’（Lilium oriental hybrid ‘Siberia’）花被片中克隆了一个衰老相关基因LoENDONUCLEASE 1（SAG10），利用qRT-PCR进行基因表达分析，并通过农杆菌介导的拟南芥稳定表达系统和百合花被片瞬时表达体系验证LoENDONUCLEASE 1基因功能。结果显示，LoENDONUCLEASE 1基因开放阅读框（ORF）为921 bp，编码306个氨基酸；LoENDONUCLEASE 1在百合花朵和叶片的衰老过程中特异表达，且受到脱落酸和水杨酸的诱导；拟南芥过表达LoENDONUCLEASE 1株系中叶片叶绿素含量显著低于对照株系，百合花被片中瞬时过表达LoENDONUCLEASE 1基因加速了百合花被片的衰老，且过表达花被片中丙二醛和离子渗透率含量显著高于对照。结果表明，LoENDONUCLEASE 1具有促进花朵和叶片衰老的功能。
A senescence-related gene LoENDONUCLEASE 1 （SAG10） was cloned from the tepals of Lilium oriental hybrid ‘Siberia’ to study the molecular mechanism underlying the senescence of flower in lily （Lilium spp.）. The expression of SAG10 was analyzed with qRT-PCR， and the function of LoENDONUCLEASE 1 gene was identified with Agrobacterium-mediated stable overexpression system of Arabidopsis thaliana and transient overexpression system of lily tepals. The results showed that the open reading frame （ORF） of LoENDONUCLEASE 1 gene was 921 bp， encoding 306 amino acids. LoENDONUCLEASE 1 was uniquely expressed in the senescence of flowers and leaves in lily， and was induced by abscisic acid and salicylic acid. The content of chlorophyll in leaves of Arabidopsis strain with overexpressing LoENDONUCLEASE 1 was significantly lower than that in the control. LoENDONUCLEASE 1 gene was transiently overexpressed in lily tepals， accelerating the senescence of tepals in lily. The content of malondialdehyde （MDA） and ion leakage rate in the overexpressed tepals were significantly higher than that in the control. It is indicated that LoENDONUCLEASE 1 can promote the senescence of flowers and leaves in lily.
引用本文
李蕊蕊,刘玉杰,许鑫彤,刘熠,何颖姣,王彩云,罗靖.核酸酶基因LoENDONUCLEASE 1在东方百合花朵衰老过程中的功能分析[J].华中农业大学学报,2024,43(1):157-165
复制
文章指标
- 点击次数:324
- 下载次数: 743
- HTML阅读次数: 207
历史
- 收稿日期:2022-10-11
- 在线发布日期: 2024-01-30
文章二维码
