首页 > 过刊浏览>2024年第43卷第1期 >149-156. DOI:10.13300/j.cnki.hnlkxb.2024.01.017
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高温胁迫对‘索邦’百合花色合成的影响
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作者:
作者单位:

华中农业大学园艺林学学院/果蔬园艺作物种质创新与利用全国重点实验室/ 华中农业大学花卉研究所，武汉 430070

作者简介:

鲁思勤，E-mail：1350441594@qq.com

通讯作者:

何燕红，E-mail：hyh2010@mail.hzau.edu.cn

中图分类号:

S682.2+65

基金项目:

国家重点研发计划项目（2020YFD1000400）；财政部和农业农村部国家现代农业产业技术体系（CARS-23-C10）


Effects of high temperature on color synthesis of flowers in oriental hybrid lily ‘Sorbonne’
Author:
  • LU Siqing

    LU Siqing

    College of Horticulture and Forestry Sciences， Huazhong Agricultural University/ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ The Institute of Flowers Research， Huazhong Agricultural University，Wuhan 430070， China
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  • DUAN Feng

    DUAN Feng

    College of Horticulture and Forestry Sciences， Huazhong Agricultural University/ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ The Institute of Flowers Research， Huazhong Agricultural University，Wuhan 430070， China
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  • ZHANG Lixue

    ZHANG Lixue

    College of Horticulture and Forestry Sciences， Huazhong Agricultural University/ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ The Institute of Flowers Research， Huazhong Agricultural University，Wuhan 430070， China
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  • NING Guogui

    NING Guogui

    College of Horticulture and Forestry Sciences， Huazhong Agricultural University/ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ The Institute of Flowers Research， Huazhong Agricultural University，Wuhan 430070， China
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  • HE Yanhong

    HE Yanhong

    College of Horticulture and Forestry Sciences， Huazhong Agricultural University/ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ The Institute of Flowers Research， Huazhong Agricultural University，Wuhan 430070， China
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Affiliation:

College of Horticulture and Forestry Sciences， Huazhong Agricultural University/ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ The Institute of Flowers Research， Huazhong Agricultural University，Wuhan 430070， China

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    摘要:

    为精准探究高温胁迫对百合开花品质的影响，以东方百合‘索邦’为试验材料，研究了不同发育阶段的花苞经过不同温度和不同时间处理后，盛花期花径、花色和矢车菊素含量的变化，以及温度处理过程中矢车菊素合成通路的3个关键基因LhCHS、LhF3H和LhANS的表达量变化。结果显示：S5－S6时期是‘索邦’百合花苞显色的关键时期；花苞在高于30 ℃温度培养4 d后，盛花期花径显著变小，矢车菊含量显著降低，花被片颜色变浅； qRT-PCR结果显示，花苞显色的关键时期，遇到30 ℃以上的高温处理导致LhCHS、LhF3H、LhANS等花青素合成基因表达量显著下调，从而影响盛花期花色的合成。

    Abstract:

    The oriental hybrid lily ‘Sorbonne’ was used to precisely study the effects of high temperature on the flowering quality of lily. The changes in flower diameter， color， and content of cyanidin at different developmental stages of flower buds treated at different temperatures and times during the period of full bloom were investigated. The changes in the expression level of three key genes involved in the synthesis pathway of cyanidin including LhCHS， LhF3H and LhANS during temperature treatment were identified as well. The results showed that the S5 and S6 stages were key period for color development of 'Sorbonne' buds. The flower diameter was significantly smaller， the content of cyaindin was significantly reduced， and the color of tepal was lighter at the stage of full bloom after the buds were treated at a temperature higher than 30 ℃ for 4 days. The results of qRT-PCR analysis showed that high temperature treatment above 30 ℃ during the critical period of flower bud coloration significantly downregulated the expression of anthocyanin synthesis genes such as LhCHS， LhF3H， and LhANS， thereby affecting the synthesis of flower color during the period of full bloom. It will provide a theoretical basis for temperature control in the production and postharvest process of lily‘Sorbonne’.

    表 1 定量引物序列Table 1 Sequences of primers for qRT-PCR
    图1 ‘索邦’百合花苞显色过程和矢车菊素含量变化Fig.1 The flower bud coloration process and the variation tendency of cyaindin content of Lilium ‘Sorbonne’
    图2 百合S2-S6时期花苞经过35 ℃处理2、4 d后盛花期的花色Fig.2 The tepal color of lily at full blossom for the flower bud from S2 to S6 with the treatment of 35 ℃ for 2，4 days
    图3 百合不同发育期的花苞经35 ℃处理2、4 d后盛花期矢车菊素含量（A）和花径（B）Fig.3 The cotent of cyanidin（A） and flower diameter（B） of lily at full blossom for the flower bud at different development stages with the treatment of 35 ℃ for 2，4 days
    图4 S3时期花苞经不同温度处理2、4、6 d后盛花期花色Fig.4 The tepal color of lily at full blossom for S3 flower buds treated with different temperature for 2，4，6 days
    图5 S3时期花苞经不同温度处理2 、4 、6 d盛花期矢车菊素含量（A）和花径（B）Fig.5 The cotent of cyanidin（A） and flower diameter（B） of lily at full blossom for S3 flower buds treated with different temperature for 2，4，6 days
    图6 不同时期花苞25 ℃和35 ℃处理2、4 d外轮花被片中LhCHS、LhF3H、LhANS的表达量Fig.6 Relative expression of LhCHS， LhF3H， LhANS gene in the outer tepal for the flower bud at different development stages treated with 25 ℃ and 35 ℃ for 2，4 days
    图7 S3百合花苞经不同温度处理2、4、6 d外轮花被片中LhCHS、LhF3H、LhANS的表达量Fig.7 Relative expression of LhCHS， LhF3H， LhANS in the outer tepal of lily for flower buds S3 treated with different temperature for 2，4，6 days
    参考文献
    [1] YAMAGISHI M,KISHIMOTO S,NAKAYAMA M.Carotenoid composition and changes in expression of carotenoid biosynthetic genes in tepals of Asiatic hybrid lily[J].Plant breeding,2010,129(1):100-107.
    [2] VEBERIC R,SLATNAR A,BIZJAK J,et al.Anthocyanin composition of different wild and cultivated berry species[J].LWT - food science and technology,2015,60(1):509-517.
    [3] YAMAGISHI M,YOSHIDA Y,NAKAYAMA M.The transcription factor LhMYB12 determines anthocyanin pigmentation in the tepals of Asiatic hybrid lilies (Lilium spp.) and regulates pigment quantity[J].Molecular breeding,2012,30(2):913-925.
    [4] 毕蒙蒙,曹雨薇,宋蒙,等.百合花色研究进展[J].园艺学报,2021,48(10):2073-2086.BI M M,CAO Y W,SONG M,et al.Advances in flower color research of Lilium[J].Acta horticulturae sinica,2021,48(10):2073-2086 (in Chinese with English abstract).
    [5] 肖伟,张铭芳,张秀海,等.百合花青素合成调控的研究进展[J].北方园艺,2018(20):154-162.XIAO W,ZHANG M F,ZHANG X H,et al.Research progress of anthocyanin synthesis regulation of lily[J].Northern horticulture,2018(20):154-162 (in Chinese with English abstract).
    [6] AN J P,ZHANG X W,YOU C X,et al.MdWRKY40 promotes wounding-induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2-mediated degradation[J].New phytologist,2019,224(1):380-395.
    [7] 胡可,韩科厅,戴思兰.环境因子调控植物花青素苷合成及呈色的机理[J].植物学报,2010,45(3):307-317.HU K,HAN K T,DAI S L.Regulation of plant anthocyanin synthesis and pigmentation by environmental factors[J].Chinese bulletin of botany,2010,45(3):307-317 (in Chinese with English abstract).
    [8] 杨捷,张星,彭梦笛,等.百合转录因子MYB12的克隆与表达分析[J].植物科学学报,2018,36(6):812-816,818.YANG J,ZHANG X,PENG M D,et al.Cloning and expression analysis of MYB12 in Lilium oriental hybrid’Sorbonne’[J].Plant science journal,2018,36(6):812-816,818 (in Chinese with English abstract).
    [9] NAKATSUKA A,YAMAGISHI M,NAKANO M,et al.Light-induced expression of basic helix-loop-helix genes involved in anthocyanin biosynthesis in flowers and leaves of Asiatic hybrid lily[J].Scientia horticulturae,2009,121(1):84-91.
    [10] YAMAGISHI M.A novel R2R3-MYB transcription factor regulates light-mediated floral and vegetative anthocyanin pigmentation patterns in Lilium regale[J/OL].Molecular breeding,2016,36(1):3[2022-11-29].https://doi.org/10.1007/s11032-015-0426-y.
    [11] LAI Y S,YAMAGISHI M,SUZUKI T.Elevated temperature inhibits anthocyanin biosynthesis in the tepals of an oriental hybrid lily via the suppression of LhMYB12 transcription[J].Scientia horticulturae,2011,132:59-65.
    [12] 张旭红,孙美玉,李靖锐,等.东方百合‘索邦’GA20ox的克隆及表达分析[J].园艺学报,2019,46(1):74-86.ZHANG X H,SUN M Y,LI J R,et al.Cloning and expression analysis of GA20ox gene in Lilium oriental hybrids ‘sorbonne’[J].Acta horticulturae sinica,2019,46(1):74-86 (in Chinese with English abstract).
    [13] YAMAGISHI M.Oriental hybrid lily Sorbonne homologue of LhMYB12 regulates anthocyanin biosyntheses in flower tepals and tepal spots[J].Molecular breeding,2011,28(3):381-389.
    [14] 刘雅莉.百合乙烯生成与衰老的关系及ACC氧化酶基因cDNA的克隆[D].杨凌:西北农林科技大学,2004.LIU Y L.The relation ethylene production to senescence and cloning of the ACC oxidase genes from lily[D].Yangling:Northwest A & F University,2004 (in Chinese with English abstract).
    [15] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method[J].Methods,2001,25(4):402-408.
    [16] 韩笑盈.菊花高温下花色褪色差异表达基因分析及CmHY5的功能研究[D].南京:南京农业大学,2020.HAN X Y.Analysis of differentially expressed genes in color fading under heat stress and functional analysis of CmHY5 in Chrysanthemum[D].Nanjing:Nanjing Agricultural University,2020 (in Chinese with English abstract).
    [17] SULLIVAN C N,KOSKI M H.The effects of climate change on floral anthocyanin polymorphisms[J/OL].Proceedings biological sciences,2021,288(1946):2020.2693[2022-11-29 ].https://doi.org/10.1098/rspb.2020.2693.
    [18] NIU J P,ZHANG G J,ZHANG W T,et al.Anthocyanin concentration depends on the counterbalance between its synthesis and degradation in plum fruit at high temperature[J/OL].Scientific reports,2017,7:7684[2022-11-29 ].https://doi.org/10.1038/s41598-017-07896-0.
    [19] KUI L W,MICHELETTI D,PALMER J,et al.High temperature reduces apple fruit colour via modulation of the anthocyanin regulatory complex[J].Plant,cell & environment,2011,34(7):1176-1190.
    [20] 张圣美.高温胁迫对茄子果皮花青素生物合成的影响[D].南京:南京农业大学,2020.ZHANG S M.Effects of high temperature stress on anthocyanin biosynthesis in eggplant peel[D].Nanjing:Nanjing Agricultural University,2020 (in Chinese with English abstract).
    [21] 洪燕红,叶清华,李泽坤,等.红花草莓‘莓红’花瓣花色苷积累及其MYB基因的表达分析[J].园艺学报,2021,48(8):1470-1484.HONG Y H,YE Q H,LI Z K,et al.Accumulation of anthocyanins in red-flowered strawberry‘Meihong’petals and expression analysis of MYB gene[J].Acta horticulturae sinica,2021,48(8):1470-1484 (in Chinese with English abstract).
    [22] FU Z Z,SHANG H Q,JIANG H,et al.Systematic identification of the light-quality responding anthocyanin synthesis-related transcripts in Petunia petals[J].Horticultural plant journal,2020,6(6):428-438.
    [23] MENG J X,GAO Y,HAN M L,et al.In vitro anthocyanin induction and metabolite analysis in Malus spectabilis leaves under low nitrogen conditions[J].Horticultural plant journal,2020,6(5):284-292.
    [24] YAMAGISHI M,SHIMOYAMADA Y,NAKATSUKA T,et al.Two R2R3-MYB genes,homologs of Petunia AN2,regulate anthocyanin biosyntheses in flower tepals,tepal spots and leaves of Asiatic hybrid lily[J].Plant and cell physiology,2010,51(3):463-474.
    [25] TIRUMALAI V,SWETHA C,NAIR A,et al.miR828 and miR858 regulate VvMYB114 to promote anthocyanin and flavonol accumulation in grapes[J].Journal of experimental botany,2019,70(18):4775-4792.
    [26] JIANG S H,WANG N,CHEN M,et al.Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple[J].Plant biotechnology journal,2020,18(8):1736-1748.
    [27] CAI H Y,ZHANG M,CHAI M N,et al.Epigenetic regulation of anthocyanin biosynthesis by an antagonistic interaction between H2A.Z and H3K4me3[J].New phytologist,2019,221(1):295-308.
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鲁思勤,段枫,张莉雪,宁国贵,何燕红.高温胁迫对‘索邦’百合花色合成的影响[J].华中农业大学学报,2024,43(1):149-156

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  • 收稿日期:2022-11-29
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