湖北地区设施栽培对‘鸡尾’葡萄柚果实品质的影响
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作者:
作者单位:

1.果蔬园艺作物种质创新与利用全国重点实验室,武汉 430070;2.武汉市农科院林业果树研究所,武汉 430075;3.武汉霄垚农业科技发展有限公司,武汉 430208

作者简介:

贺晨菲,E-mail:1403923568@qq.com

通讯作者:

刘继红,E-mail:liujihong@mail.hzau.edu.cn

中图分类号:

S666.3

基金项目:

湖北省重点研发专项(2022BBA0073);湖北省特色水果创新行动计划项目


Effects of facility cultivation on fruit quality of ‘Cocktail’ grapefruit in Hubei Province
Author:
Affiliation:

1.National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Wuhan 430070, China;2.Forestry and Fruit Tree Research Institute,Wuhan Academy of Agricultural Sciences, Wuhan 430075, China;3.Wuhan Xiaoyao Agricultural Science and Technology Development Co., Ltd., Wuhan 430208, China

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    摘要:

    为提升湖北地区‘鸡尾’葡萄柚品质,以‘鸡尾’葡萄柚为材料,研究设施栽培和露天栽培(CK)模式下果实品质的差异。结果显示:设施栽培下‘鸡尾’葡萄柚果实单果质量、横纵径、果形指数大于CK,果皮硬度在果实发育过程中差异不显著,花后227 d设施栽培和露天栽培果皮硬度出现显著性差异;设施栽培可显著提高果实可溶性固形物含量至10.43%,可滴定酸、Vc含量高于露天栽培;通过GC-MS测定发现,设施栽培可提升果实蔗糖含量至49.63 mg/g;实时荧光定量PCR分析发现设施栽培可能通过上调CsSPS2、CsSUS2和CsSUS4的表达水平来提高果实中蔗糖的含量。结果表明设施栽培可以提高‘鸡尾’葡萄柚的果实品质。

    Abstract:

    The differences in fruit quality of ‘Cocktail’ grapefruit between the mode of facility cultivation and open cultivation (CK) were studied to improve the quality of ‘Cocktail’ grapefruit in Hubei Province. The results showed that the single fruit weight, transverse and longitudinal diameter and fruit shape index of facility cultivation were larger than those of CK. There was no significant difference in peel hardness during fruit development. There was a significant difference in peel hardness between facility cultivation and CK from 227 days after anthesis. Facility cultivation significantly increased the content of soluble solid in fruits to 10.43%. The content of titratable acid and Vc in facility cultivation was higher than that in CK. The results of GC-MS analysis showed that facility cultivation increased the content of sucrose in fruits to 49.63 mg/g. The results of real-time quantitative PCR analysis showed that facility cultivation increased the content of sucrose in fruits by upregulating the expression levels of CsSPS2CsSUS2 and CsSUS4. It is indicated that facility cultivation can improve the fruit quality of ‘Cocktail’ grapefruit.

    表 2 不同栽培模式下‘鸡尾’葡萄柚果实品质变化Table 2 Changes in fruit quality of ‘Cocktail’ grapefruit under different cultivation modes
    表 1 定量分析所用引物Table 1 List of primers for quantitative real-time PCR
    图1 不同栽培模式下果实外观变化Fig.1 Changes of fruit appearance under different cultivation modes
    图2 不同栽培模式对‘鸡尾’葡萄柚果实品质的影响Fig.2 Effects of different cultivation modes on fruit quality of ‘Cocktail’ grapefruit
    图3 不同栽培模式下‘鸡尾’葡萄柚果实可溶性糖及有机酸含量Fig.3 Content of soluble sugars and organic acids in grapefruit under different cultivation modes
    图4 不同栽培模式对‘鸡尾’葡萄柚果实蔗糖代谢相关基因的影响Fig.4 Effects of different cultivation modes on genes related to sucrose metabolism in ‘Cocktail’ grapefruit fruit
    图5 不同栽培模式下‘鸡尾’葡萄柚柠檬酸相关代谢基因表达水平的变化Fig.5 Change of different cultivation patterns of citric acid-related metabolic genes expression of Cocktail grapefruit
    图6 设施栽培对温度的影响Fig.6 Effect of protected cultivation on temperature
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引用本文

贺晨菲,宿福园,庞在虎,方林川,裴忺,王燕,李长林,刘继红.湖北地区设施栽培对‘鸡尾’葡萄柚果实品质的影响[J].华中农业大学学报,2024,43(1):124-132

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  • 收稿日期:2022-12-09
  • 在线发布日期: 2024-01-30
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