红橘×枳杂交子代遗传多样性分析
作者:
作者单位:

1.果蔬园艺作物种质创新与利用全国重点实验室/华中农业大学园艺林学学院,武汉430070;2.广东省农业科学院德庆县柑橘研究所,德庆526600

作者简介:

孙怡,E-mail: 1163343923@qq.com

通讯作者:

谢宗周,E-mail: xiezz@webmail.hzau.edu.cn

中图分类号:

S666

基金项目:

广东省重点研发项目(2022B0202070002); 国家自然科学基金项目 (32122075,32072523); 国家现代农业(柑橘)产业技术体系(CARS-26)


Genetic diversity analysis of hybrid progeny of Red tangerine ×Trifoliate orange
Author:
Affiliation:

1.National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops/ College of Horticulture & Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;2.Deqing Citrus Research Institute,Guangdong Academy of Agricultural Sciences,Deqing 526600,China

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    摘要:

    为了筛选集双亲优异性状的优良砧木,以柑橘主要砧木红橘(♀)×枳(♂)杂交F1代94个单株为试材,连续2 a对其落叶期及花期进行观测,并对结果率较高的37个单株的果实品质、种子单多胚性及萌发率等进行分析。结果显示:94个单株物候期与双亲相近,但单株间落叶期差异明显;子代群体果形更偏向于父本遗传,主要以卵圆形为主;子代群体平均单果质量为58.63 g,平均单果种子数为14粒,平均千粒重为153.73 g;果实可溶性固形物在10.7% ~ 16.5%,与母本无显著性差异,可滴定酸含量在2.00% ~ 5.56%,显著高于母本;果皮色泽主要有红色(偏向母本)和黄色(偏向父本)2种,子代群体中β-隐黄质的含量显著高于双亲,紫黄质、叶黄质和玉米黄质的含量介于双亲之间,而β-胡萝卜素在亲本和杂交后代果皮中含量则较低,其中紫黄质、叶黄质和β-胡萝卜素等物质含量呈现连续变化,具有数量性状特征;群体子代种子单胚与多胚类型接近1∶3,符合单基因控制模型。结果表明,子代单株3号、6号及24号兼具多胚、单果种子数多及萌发率高等特性,可作为候选优异砧木资源做进一步评价。

    Abstract:

    To screen the rootstock resources for superior traits of convergent parents,94 individual plants of F1 hybrid population with Red tangerine as the female parent and Trifoliate orange as the male parent were used as test materials to observe their defoliation period and flowering period for two consecutive years,and the fruit quality,seed embryo types and germination rate of 37 individual plants with high fruition rates were analyzed. The results showed that the phenology of 94 individual plants was similar to that of their parents,but there was a significant difference in the defoliation period between hybrid progenies. The fruit shape of the hybrid population was more inclined toward paternal inheritance,mainly oval in shape. The average fruit weight of the offspring populations was 58.63 g,average number of seeds was 14,and 1000-grain weight was 153.73 g.The soluble solid content was 10.7%?16.5%,which was not significantly different from that of the female parent,and the titratable acid content of fruit was 2.00%?5.56%,which was significantly higher than that of the female parent. The flavedo of the hybrid population mainly includes red peel (female parent preferred) and yellow peel (male parent preferred). The contents of β-cryptoxanthin were significantly higher than those of the parents,and the contents of violaxanthin lutein and zeaxanthin were between those of parents,while the contents of β-carotene in the peel of parents and hybrid offspring were relatively low. The contents of violaxanthin,lutein and β-carotene showed continuous changes,which showed quantitative characteristics. The ratio of monoembryoid to polyembryoid types in the hybrid progeny was close to 1∶3,which indicated that the character was determined by a single gene. In summary,the results showed that the progeny No. 3,No. 6 and No. 24 had the characteristics of polyembryony ,more seeds per fruit and a high germination rate,and could be used as excellent rootstock candidates for further evaluation.

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孙怡,胡益波,叶俊丽,柴利军,邓秀新,谢宗周.红橘×枳杂交子代遗传多样性分析[J].华中农业大学学报,2023,42(4):150-157

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  • 收稿日期:2022-09-03
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  • 在线发布日期: 2023-08-30
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