1.沈阳农业大学生物科学技术学院，沈阳110866;2.辽宁省林业科学研究院，沈阳110032
林景卫，E-mail： 2009500003@syau.edu.cn
S567.3+9
辽宁省教育厅科学研究基础项目（LSNQN202013）陈焕，E-mail：2507297357@qq.com
- CHEN Huan 1
- FAN Tianning 1
- BAI Yudong 1
- GUO Qianqian 1
- WANG Haoan 1
- CHEN Gang 2
- GAO Yingxu 2
- LIN Jingwei 1
1.College of Biological Science and Technology， Shenyang Agricultural University， Shenyang 110866，China;2.Liaoning Academy of Forestry Sciences，Shenyang 110032，China
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为探究FIP-fve基因在金针菇本体的生物学功能，构建了pCAMBIA1301-pGPD-FIP-fve超表达载体并将其转化至农杆菌LBA4404中，采用农杆菌介导的金针菇转化法，最终获得了FIP-fve基因超表达的金针菇；进行FIP-ve基因沉默（RNAi： FIP-fve）、FIP-fve超表达（pGPD： FIP-fve）和野生金针菇（CK）菌丝体培养和子实体栽培，并记录菌丝体和子实体时期的生物学性状。结果显示：pGPD： FIP-fve金针菇菌丝生长速度最快，CK金针菇次之，而RNAi： FIP-fve金针菇生长速度最为缓慢；子实体出菇栽培后，在子实体数量、菌柄长度、子实体产量和生物学效率方面，pGPD： FIP-fve金针菇都明显优于CK金针菇，而RNAi： FIP-fve金针菇的子实体生长状况最差。研究结果表明，FIP-fve基因能够促进金针菇菌丝体的生长，并对金针菇原基形成具有促进作用，最终影响金针菇的产量。
FIP-fve is a low molecular-mass protein identified from Flammulina filiformis， which has many biological activities including immunomodulation. Our previous studies found that FIP-fve had certain growth-promoting effect on F. filiformis mycelia. We constructed an over-expression vector pCAMBIA1301-pGPD-FIP-fve， and transformed it into Agrobacterium tumefaciens LBA4404 strain to study the native biological function of FIP-fve to F. filiformis. FIP-fve gene overexpressed F. filiformis was obtained via Agrobacterium transformation. By comparing the biological characteristics of FIP-fve gene-silent F. filiformis （RNAi：FIP-fve）， FIP-fve gene-overexpressed F. filiformis （pGPD：FIP-fve） and wild F. filiformis （CK） in mycelium and fruiting body stages， we found that the growth speed of pGPD：FIP-fve was faster than that of CK， whereas the growth speed of RNAi：FIP-fve was the lowest. After cultivating the fruiting body， yield-related traits of pGPD：FIP-fve were significantly better than that of CK in terms of fruiting body numbers， stalk length， fruiting body yield and biological efficiency， and those traits of RNAi： FIP fve were the worst. It is indicated that FIP-fve significantly promote the growth of F. filiformis mycelia and affect the final yields of F. filiformis.
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陈焕,范天宁,白玉东,郭芡芡,王浩安,陈罡,高英旭,林景卫.金针菇中免疫调节蛋白编码基因FIP-fve的功能[J].华中农业大学学报,2023,42(2):131-138
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- 收稿日期:2022-08-03
- 在线发布日期: 2023-03-31
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