Nanjing 9108 and Huanghuazhan were used as materials under machine direct seeding. Under total nitrogen 210 kg/hm² in the field， three different nitrogen fertilizer treatments including N1 （base fertilizer 5∶tiller fertilizer 2∶young spike fertilizer 3）， N2 （3∶4∶3） and N3 （none∶6∶4） were set up， and no nitrogen fertilizer （N0） was used as the control to study the effects of different nitrogen management on the yield， quality and nitrogen fertilizer utilization efficiency of rice with machine direct seeding. The results showed that the yield of the two rice varieties was the highest when the fertilization ratio was 3∶4∶3. The average yield of Nanjing 9108 and Huanghuazhan was as high as 9 691.2 kg/hm² and 11 596.1 kg/hm²， significantly different from that of other treatments. The brown rice rate， milled rice rate and head rice rate of rice were not significantly different among treatments， but the content of amylose and protein increased with the increase of panicle fertilizer ratio in the decreasing order of N3 > N2 > N1. The chalkiness rate and chalkiness decreased with the increase of panicle fertilizer ratio in the decreasing order of N1 > N2 > N3. The rice quality of N3 （none∶6∶4 ） was significantly higher than that of other treatments. The nitrogen absorption of rice at stage of each growth under the treatment of 3∶4∶3 was significantly higher than that of other treatments. The nitrogen agronomic efficiency and nitrogen use efficiency were N2 > N3 > N1， reaching a significant level. It is indicated that， N2 （3∶4∶3） significantly affect the yield and nitrogen use efficiency of rice under the same nitrogen application rate， and N3 （ none∶6∶4 ） significantly affect the rice quality.