To evaluate the dynamics of antibodies production after subcutaneous and intradermal inoculation of attenuated goat pox vaccine （AV41 strain） in cattle and optimize the immunization program of cattle，25 healthy beef cattle were randomly assigned into 5 groups and inoculated subcutaneously and intradermally at 5 and 10 times of goat dose，respectively.The standard single dose was 1.0 × 10^3.5 TCID₅₀ AV41 strain.At 0，15，30，45 and 60 days post vaccination，the serum antibody level was detected by both virus neutralisation test （VNT） and a commercial ELISA kit.The PCR results of nasopharyngeal swabs and blood were negative without shedding vaccine strain.The results of VNT and ELISA antibody detection were similar，60% of the cattle in the subcutaneous inoculation with 5 times dose group seroconverted on day 30 post vaccination，with the maximum neuralising antibody titer of 1∶640，followed by the intradermal inoculation with 10 times dose group and subcutaneous inoculation with 10 times dose group （40%），while only 20% seroconverted in the intradermal inoculation with 5 times dose group.The neutralisation index of VNT was significantly positively correlated with the S/P value of ELISA.The above results suggest that similar to the inoculation route and dose of relevant international vaccines，subcutaneous inoculation of AV41 at a 5 times goat dose is safe and effective and can be used as a clinical vaccination protocol for lumpy skin disease.