首页 > 过刊浏览>2023年第42卷第1期 >66-74. DOI:10.13300/j.cnki.hnlkxb.2023.01.009
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水涝胁迫对草石蚕生理特性的影响及抗涝性评价
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作者单位:

1.贵州省农业科学院园艺研究所,贵阳 550006;2.贵州省园艺工程技术研究中心,贵阳550006;3.贵州大学农学院,贵阳 550025

作者简介:

班甜甜，E-mail：1574601087@qq.com

通讯作者:

耿广东，E-mail： genggd213@163.com

中图分类号:

S644.501

基金项目:

国家自然科学基金项目（31760596）； 国家自然科学基金补助项目（黔农科院国基后补助［2021］22号）；贵州省蔬菜现代农业产业技术体系（GZCYTX2021-01）；贵州省农业科学院专项（黔农科院种质资源（2020）10号）；贵州省科技计划项目（黔科合成果［2021］一般063）


Effects of waterlogging stress on physiological characteristics and evaluation of waterlogging resistance of Stachys sieboldii Miq.
Author:
Affiliation:

1.Horticultural Institute,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;2.Guizhou Province Research Center for Horticulture Engineering Technology,Guiyang 550006,China;3.College of Agronomy,Guizhou University,Guiyang 550025,China

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    摘要:

    以3个草石蚕（Stachys sieboldii Miq.）品种为试材，研究水涝胁迫对草石蚕生理特性的影响，并通过相关性和主成分分析，对草石蚕的抗涝性进行评价，以期为草石蚕抗涝性鉴定提供参考。结果显示，随水涝胁迫时间延长，3种草石蚕叶绿素、可溶性糖、可溶性蛋白含量及超氧化物歧化酶（POD）和过氧化氢酶（CAT）活性呈先升高后降低的变化；“鲁引1号”和“赣引1号”的脯氨酸（Pro）含量先升高后降低，“贵栽1号”Pro含量则一直升高；“鲁引1号”和“贵栽1号”的SOD活性先升高后降低，“赣引1号”则一直升高；随胁迫时间的延长，3种草石蚕的MDA含量均一直增加。相关性分析发现草石蚕的叶绿素含量、可溶性糖含量、Pro含量、SOD活性、POD活性、CAT活性和MDA含量间具有密切的相关性，但相关程度不一致。基于主成分分析的抗涝性综合评价，3种草石蚕的抗涝性为赣引1号>鲁引1号>贵栽1号。

    Abstract:

    Three Stachys sieboldii Miq.varieties were used to study the effects of waterlogging stress on the physiological characteristics of S. sieboldii Miq..Correlation analysis and principal component analysis were used to evaluate the waterlogging resistance of S. sieboldii Miq. to provide a reference for the identification of waterlogging resistance of S. sieboldii Miq..The results showed that the content of chlorophyll，soluble sugar，soluble protein，and the activity of POD and CAT of the three S. sieboldii Miq.varieties increased first and then decreased with the prolongation of waterlogging stress.The content of Pro in Luyin No.1 and Ganyin No.1 first increased and then decreased，while the content of Pro in Guizai No.1 kept increasing.The activity of SOD in Guizai NO.1 and Luyin No.1 increased first and then decreased，while the activity of SOD in Ganyin No.1 kept increasing under the waterlogging stress.The accumulation of MDA in the three S. sieboldii Miq. varieties increased all the time with the extension of stress time.The results of correlation analyses showed that the content of chlorophyll，soluble sugar，soluble protein，Pro，and MDA，the activity of SOD，POD，and CAT of S. sieboldii Miq.was closely related，but the degree of correlation was inconsistent.The results of comprehensively evaluating waterlogging resistance based on the principal component analysis showed that the waterlogging resistance of the three S. sieboldii Miq. varieties was in the decreasing order of Ganyin No.1>Luyin No.1> Guizai No.1.

    表 6 以农业收入占比分类的异质性分析Table 6
    表 1 草石蚕各生理指标间的相关性分析Table 1 Correlation analysis of Stachys sieboldii Miq. physiological indexes
    表 5 以家庭年收入分类的异质性分析Table 5
    表 4 草石蚕主成分得分及综合评价Table 4 Principal component score and comprehensive evaluation value of S. sieboldii Miq.
    图1 多维视角下农户施肥行为分析的理论框架Fig.1
    图1 水涝胁迫下草石蚕叶绿素含量的变化Fig.1 The change of chlorophyll content of Stachys sieboldii Miq. under waterlogging stress
    图2 水涝胁迫下草石蚕可溶性糖含量的变化Fig.2 The change of soluble sugar content of Stachys sieboldii Miq. under waterlogging stress
    图3 水涝胁迫下草石蚕可溶性蛋白含量的变化Fig.3 The change of soluble protein content of Stachys sieboldii Miq. under waterlogging stress
    图4 水涝胁迫下草石蚕脯氨酸含量的变化Fig.4 The change of proline content of Stachys sieboldii Miq. under waterlogging stress
    图5 水涝胁迫下草石蚕SOD活性的变化Fig.5 The change of SOD activity of Stachys sieboldii Miq. under waterlogging stress
    图6 水涝胁迫下草石蚕POD活性的变化Fig.6 The change of POD activity of Stachys sieboldii Miq. under waterlogging stress
    图7 水涝胁迫下草石蚕CAT活性的变化Fig.7 The change of CAT activity of Stachys sieboldii Miq. under waterlogging stress
    图8 水涝胁迫下草石蚕MDA含量的变化Fig.8 The changeof MDA content of Stachys sieboldii Miq. under waterlogging stress on
    表 3 主成分得分系数Table 3 The matrix of principal component scoring coefficient
    表 2 各成分特征值和贡献率Table 2 The characteristic value and contribution rate of each component
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班甜甜,张素勤,马超,吴传递,徐彦军,李晓慧,耿广东.水涝胁迫对草石蚕生理特性的影响及抗涝性评价[J].华中农业大学学报,2023,42(1):66-74

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  • 收稿日期:2022-04-28
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